Product Description for Cytokeratin 7
Guinea Pig anti Human Cytokeratin 7.
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.
Properties for Cytokeratin 7
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin |
| Presentation | Serum |
| Reactivity | Hu |
| Applications | C, ICC/IF, P, WB |
| Clonality | Polyclonal |
| Host | Guinea Pig |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Application | Western blotting: 1/3000 (ECL). Immunohistochemistry on frozen Sections: 1/200. Immunohistochemistry on paraffin-embedded: 1/100 (after microwave treatment). Cytological material. Incubation time: 1 h at RT; extended with paraffin sections. Positive control: Glandular epithelia (e.g. sweat glands, sebaceous glands); Merkel cells. |
| Background | Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19. |
| General Readings | Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982). Ramaekers, F., van Niekerk C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G. and Vooijs, P.: Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am. J. Pathol. 136, 641-655 (1990) |
| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 3855 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cytokeratin 7 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00003855-P01-10 |
Cytokeratin 7 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003855-P01-25 |
Cytokeratin 7 |
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|
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cytokeratin 7 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-12396 |
Cytokeratin 7 Lysate |
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![Western Blot: Cytokeratin 7 Lysate [NBL1-12396] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KRT7](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin%207%20Lysate-Western%20Blot-NBL1-12396-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| H00003855-T01 |
KRT7 293T Cell Transient Overexpression Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
| LY401707 |
KRT7 overexpression lysate |
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0.1 mg /
€295.00
|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for Cytokeratin 7 (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1322AP |
Guinea pig IgG (H+L chain) antibody |
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|
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Rabbit | AP | |||||
| OriGene Technologies GmbH | |||||||
| R1322F |
Guinea pig IgG (H+L chain) antibody |
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|
|
Rabbit | FITC | |||||
| OriGene Technologies GmbH | |||||||
| R1322HRP |
Guinea pig IgG (H+L chain) antibody |
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|
|
Rabbit | HRP | GP | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1322P |
Guinea pig IgG (H+L chain) antibody |
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|
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Rabbit | Aff - Purified | |||||
| OriGene Technologies GmbH | |||||||
| R1322T |
Guinea pig IgG (H+L chain) antibody |
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|
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Rabbit | TRITC | |||||
| OriGene Technologies GmbH | |||||||
| R1436C2 |
Guinea pig IgG (H+L chain, adsorbed) antibody |
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|
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Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1436C3 |
Guinea pig IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1436C5 |
Guinea pig IgG (H+L chain), adsorbed antibody |
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|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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