Interleukin-1 alpha / IL-1A - DDX0710B-50

Product Description for Interleukin-1 alpha / IL-1A

Human anti Human Interleukin-1 alpha / IL-1A HuMab X3.
Presentation: Biotin

Properties for Interleukin-1 alpha / IL-1A

Product Category	Primary Antibodies
Quantity	50 µg
Synonyms	Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
Presentation	Biotin
Reactivity	Hu
Clonality	Monoclonal
Clone	HuMab X3
Host	Human
Isotype	IgG4
Shipping to	Germany, USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Dendritics SAS

Datasheet Extract

Immunogen	Swiss Prot Num: P01583 Immunogen: CD40 activated and EBV immortalized human B lymphocytes. GeneID: 3552
Application	DDX0710P-50 / DDX0710P-100 Purified: Inhibition, Neutralization (Binding to IL1α and not to IL1 or IL1Ra, Neutralizing biological effects of natural and recombinant IL1α, Inhibition of IL1α binding to IL1 receptors. DDX0710A488-50 / DDX0710A488-100 Alexa-fluor®488 (on request). DDX0710A546-50 / DDX0710A546-100 Alexa-fluor®546 (on request). DDX0710A647-50 / DDX0710A647-100 Alexa-fluor®647 (on request). DDX0710B-50 / DDX0710B-100 Biotin (on request). Usage recommendation: This monoclonal antibody may be used between 5-25 μg/ml. Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Background	Interleukin-1 (IL-1) defines two polypeptides, IL-1α and IL-1β, that possess a wide spectrum of biological effects. Two natural antagonists of IL-1 action have been characterized: the IL-1 receptor antagonist (IL-1Ra) and a soluble form of the type II IL-1 receptor. Neutralizing autoantibodies to IL-1α have also been detected in sera of healthy individuals and patients with autoimmune or inflammatory diseases. By combining CD40-activation and Epstein-Barr virusimmortalization of B lymphocytes from individuals with circulating anti-IL-1α, a natural anti-IL- 1α monoclonal autoantibody was developed that bound specifically to human IL-1α, but not to IL- 1β and IL-1Ra, with a high affinity (Kd = 1.2 × 10⁻¹⁰ M). HuMAb X3 inhibited IL-1α binding to IL-1 receptors and neutralized biological activities of both recombinant and natural forms of IL-1α. This high affinity neutralizing anti-IL-1α human autoantibody can function as a third type of IL-1 antagonist. (Garrone P et al, Mol Immunol 1996, 33 (7-8), 649-58 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
Concentration	0.5 mg/ml
General Readings	Garrone P, 1996 abstract Guillot-Chene P et al, 2009
Storage	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Format	Label: Biotin Purification: QMA Hyper D ion exchange chromatography Buffer System: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
Specificity	Specificity: Human IL1α (binds to Human IL1α but not to IL1β and IL1Ra).

Accessory Products

Proteins and/or Positive Controls

Proteins for Interleukin-1 alpha / IL-1A (24 products)

Catalog No.	Species	Pres.	Purity	Source
TP720056	Interleukin-1 alpha / IL-1A
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / €299.00
					OriGene Technologies, Inc.
TP723206	Interleukin-1 alpha / IL-1A
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / €230.00
					OriGene Technologies, Inc.
TP723707	Interleukin-1 alpha / IL-1A
	Human		> 98 % Preparation: . Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE.	E. coli	10 µg / €265.00
					OriGene Technologies, Inc.
AR09045PU-N	Interleukin-1 alpha / IL-1A (113-271, His-tag)
	Human	Purified	> 90 % > 90% by SDS PAGE	E. coli	50 µg / €320.00
					OriGene Technologies GmbH
AR09045PU-S	Interleukin-1 alpha / IL-1A (113-271, His-tag)
	Human	Purified	> 90 % > 90% by SDS PAGE	E. coli	0.25 mg / €820.00
					OriGene Technologies GmbH
PA073	Interleukin-1 alpha / IL-1A
	Human		> 98 % pure by SDS-PAGE and HPLC analyses	E. coli
					OriGene Technologies GmbH
PA073X	Interleukin-1 alpha / IL-1A
	Human		> 98 % pure by SDS-PAGE and HPLC analyses	E. coli
					OriGene Technologies GmbH
H00003552-P01-10	Interleukin-1 alpha / IL-1A
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00003552-P01-25	Interleukin-1 alpha / IL-1A
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00003552-Q01-10	Interleukin-1 alpha / IL-1A
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00003552-Q01-25	Interleukin-1 alpha / IL-1A
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P3627	Interleukin-1 alpha / IL-1A
	Human
					Abnova Taiwan Corp.
P4402	Interleukin-1 alpha / IL-1A
	Human
					Abnova Taiwan Corp.
NBC1-18493	Interleukin-1 alpha / IL-1A
			Protein
					Novus Biologicals Inc.
NBC1-21341	Interleukin-1 alpha / IL-1A
			Affinity purified
					Novus Biologicals Inc.
NBC1-21341-0.050mg	Interleukin-1 alpha / IL-1A
			Affinity purified
					Novus Biologicals Inc.
NBC1-21341SS	Interleukin-1 alpha / IL-1A
			Affinity purified
					Novus Biologicals Inc.
NBP1-42929-0.1	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.
NBP1-42929-0.5	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.
NBP1-42932-0.1	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.
NBP1-42932-0.5	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.
NBP1-43231-5	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.
NBP1-43231-25	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.
NBP1-94207	Interleukin-1 alpha / IL-1A
	Human	Purified
					Novus Biologicals Inc.