Product Description for CD283 / TLR3
Mouse anti Human CD283 / TLR3 1210F1 / TLR3.2.
Properties: (LRR20)
Presentation: Alexa Fluor 546
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties: (LRR20)
Presentation: Alexa Fluor 546
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.
Properties for CD283 / TLR3
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | Toll-like receptor 3 |
| Presentation | Alexa Fluor 546 |
| Reactivity | Hu |
| Applications | F, ICC/IF, IP, WB |
| Clonality | Monoclonal |
| Clone | 1210F1 / TLR3.2 |
| Host | Mouse |
| Isotype | IgG1 |
| Molecular weight | 135KDa, 130KDa, 60KDa |
| Shipping to | Germany, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Dendritics SAS |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | LRR20 |
| Application | FACS intracellular, ImmunoFluorescence, Immunoprecipitation, Western Blot. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using. |
| Background | The Toll-like receptor (TLR) family are considered as key for sensing microbial infections and triggering inflammatory and innate responses. 3 novel mAbs were generated against TLR3 that support the emergence of an alternative model for TLR3 biology: TLR3 full-length (TLR3FL, 130KDa) corresponds to the TLR3 present in ER, which in resting cells is steadily converted within the Golgi into fully glycosylated TLR3 (TLR3FL+, 135KDa) and then rapidly cleaved by cathepsins in the endosome/lysosome into 2 long-lived associated fragments (72 KDa and 60KDa). (Toscano F et al, J. Immunol, 2013, 190: 764–773). |
| Concentration | 0.5 mg/ml |
| Product Citations |
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| General Readings | |
| Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
| Format |
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| Specificity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for CD283 / TLR3 (6 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP723450 |
CD283 / TLR3 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
25 µg /
€230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR31102PU-N |
CD283 / TLR3 |
||||||
|
|
Human | Purified | > 95 % pure by SDS-PAGE and HPLC analyses | HEK293 cells | |||
| OriGene Technologies GmbH | |||||||
| AR31102PU-S |
CD283 / TLR3 |
||||||
|
|
Human | Purified | > 95 % pure by SDS-PAGE and HPLC analyses | HEK293 cells | |||
| OriGene Technologies GmbH | |||||||
| H00007098-G01 |
CD283 / TLR3 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| H00007098-Q01-10 |
CD283 / TLR3 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00007098-Q01-25 |
CD283 / TLR3 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for CD283 / TLR3 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY418801 |
TLR3 overexpression lysate |
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|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for CD283 / TLR3 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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