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AR31188PU-N BMP2 / BMP2A (CHO cell derived)

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10 µg / €250.00
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Quick Overview

BMP2 / BMP2A

AR31188PU-N

Product Description for BMP2 / BMP2A

BMP2 / BMP2A.
Presentation: Purified

Properties for BMP2 / BMP2A

Product Category Proteins & Growth Factors
Quantity 10 µg
Synonyms BMP-2, BMP-2A, Bone morphogenetic protein 2
Presentation Purified
Molecular weight 26.0 kDa
Source CHO cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P12643
Purity > 95 % pure by SDS-PAGE gel and HPLC analyses.
Add. information Centrifuge the vial prior to opening!
Background BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues, including lung, liver, spleen, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains (monomers) linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.
General Readings
  1. Lyssiotis CA, Walker J, Wu C, Kondo T, Schultz PG, Wu X. Inhibition of histone deacetylase activity induces developmental plasticity in oligodendrocyte precursor cells. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14982-7. Epub 2007 Sep 12. PubMed PMID: 17855562. (Free PMC Article available, 3 images available)
  2. Chung YI, Ahn KM, Jeon SH, Lee SY, Lee JH, Tae G. Enhanced bone regeneration with BMP-2 loaded functional nanoparticle-hydrogel complex. J Control Release. 2007 Aug 16;121(1-2):91-9. Epub 2007 Jun 2. PubMed PMID: 17604871.
  3. Huang MS, Morony S, Lu J, Zhang Z, Bezouglaia O, Tseng W, et al. Atherogenic phospholipids attenuate osteogenic signaling by BMP-2 and parathyroid hormone in osteoblasts. J Biol Chem. 2007 Jul 20;282(29):21237-43. Epub 2007 May 22. PubMed PMID: 17522049. (Free PMC Article available, 6 images available)
  4. Giuliani N, Morandi F, Tagliaferri S, Lazzaretti M, Bonomini S, Crugnola M, et al. The proteasome inhibitor bortezomib affects osteoblast differentiation in vitro and in vivo in multiple myeloma patients. Blood. 2007 Jul 1;110(1):334-8. Epub 2007 Mar 19. PubMed PMID: 17371942.
  5. Wong CE, Paratore C, Dours-Zimmermann MT, Rochat A, Pietri T, Suter U, et al. Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin. J Cell Biol. 2006 Dec 18;175(6):1005-15. Epub 2006 Dec 11. PubMed PMID: 17158956. (Free PMC Article available, 8 images available)
  6. Chou YT, Yang YC. Post-transcriptional control of Cited2 by transforming growth factor beta. Regulation via Smads and Cited2 coding region. J Biol Chem. 2006 Jul 7;281(27):18451-62. Epub 2006 May 4. PubMed PMID: 16675452.
  7. Yamashita A, Takada T, Narita J, Yamamoto G, Torii R. Osteoblastic differentiation of monkey embryonic stem cells in vitro. Cloning Stem Cells. 2005;7(4):232-7. PubMed PMID: 16390259.
  8. Giuliani N, Colla S, Morandi F, Lazzaretti M, Sala R, Bonomini S, et al. Myeloma cells block RUNX2/CBFA1 activity in human bone marrow osteoblast progenitors and inhibit osteoblast formation and differentiation. Blood. 2005 Oct 1;106(7):2472-83. Epub 2005 Jun 2. PubMed PMID: 15933061.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human BMP-2 derived from CHO cells is a homodimeric glycoprotein that consists of two 114 amino acid polypeptide chains linked by a single disulfide bond. Due to glycosylation, CHO cell-derived Human BMP-2 migrates at an apparent molecular weight of approximately 28-29 kDa by SDS-PAGE analysis under non-reducing conditions.
Mature sequence is complete identical with Mouse and Rat.
AA Sequence:
QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
Biological activity:
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 40-100 ng/ml.
Molecular weight:
(2x 114 amino acids)
Format
Endotoxin level:
< 0.1 ng/µg (1EU/µg)
Purity:
>95% pure by SDS-PAGE gel and HPLC analyses.
State:
Lyophilized (sterile filtered) purified protein
Purified
Reconstitution:
Restore in water to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
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