Apolipoprotein A I / APO AI (25-264, His-tag) - AR51878PU-N

Product Description for Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI.
Presentation: Purified

Immunogen	Swiss Prot Num: Q00623
Purity	> 90 % by SDS - PAGE
Background	Apoa1 also known as Apolipoprotein A-I, is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apoa1 are associated with HDL deficiency including Tangier disease. Recombinant mouse Apoa1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Concentration	1.0 mg/ml (determined by bradford assay)
General Readings	Sontag TJ., et al. (2014) Arterioscler Thromb Vasc Biol, 34(2), 262-9.
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	AA Sequence: `MGSSHHHHHH SSGLVPRGSH MGSDEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ` Molecular weight: (263aa) confirmed by MALDI-TOF
Format	Buffer System: Liquid, In Phosphate buffered saline (pH7.4) containing 20% glycerol, 1mM DTT. Purity: >90% by SDS - PAGE State: Liquid purified protein Purified