Cytokeratin 19 - DM188 | acris-antibodies.com

Product Description for Cytokeratin 19

Mouse anti Human Cytokeratin 19 RCK108.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 19

Product Category	Primary Antibodies
Quantity	1 ml
Synonyms	CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19
Presentation	Supernatant
Reactivity	Hu
Applications	C, ICC/IF, P, WB
Clonality	Monoclonal
Clone	RCK108
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P08727 Immunogen: Human bladder cell line T24 GeneID: 3880
Application	Western Blot. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: No proteolytic pretreatment required, Optimal results are obtained when using the microwave retrieval method in citrate buffer. Working Dilution: 1/10-1/20 (ABC Method) for Immunohistochemistry. Dilution Buffer: Dilute immediately with PBS or TBS. Positive Control: Glandular epithelia.
Background	Keratin-19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.
General Readings	Kwaspen FH, Smedts FM, Broos A, Bulten H, Debie WM, Ramaekers FC. Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis. Histopathology. 1997 Dec;31(6):503-16. PubMed PMID: 9447381. Schaafsma HE, Ramaekers FC. Cytokeratin subtyping in normal and neoplastic epithelium: basic principles and diagnostic applications. Pathol Annu. 1994;29 Pt 1:21-62. PubMed PMID: 7510383. Smedts F, Ramaekers F, Robben H, Pruszczynski M, van Muijen G, Lane B, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990 Mar;136(3):657-68. PubMed PMID: 1690513. (Free PMC Article available)
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide Supernatant
Species Reactivity	Species reactivity (tested): Human, most Mammals. Negative with Mouse and Rat.
Specificity	Specificity: This antibody specifically reacts with keratin K19 (formerly also designated cytokeratin 19; 40 kD protein in immunoblotting), found in most glandular simple type epithelia and tumors derived therefrom (adenocarcinoma), most squamous cell carcinoma.
Gene ID	3880

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 19 (12 products)

Catalog No.	Species	Pres.	Purity	Source
TP309707	Cytokeratin 19
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP710256	Cytokeratin 19 (full length, C-term DDK tag)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	SF9 cells	20 µg / €411.00
					OriGene Technologies, Inc.
TP760194	Cytokeratin 19 (full length, N-term HIS tag)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
					OriGene Technologies, Inc.
AR50763PU-N	Cytokeratin 19 (1-400, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.5 mg / €820.00
					OriGene Technologies GmbH
AR50763PU-S	Cytokeratin 19 (1-400, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.1 mg / €320.00
					OriGene Technologies GmbH
BA1016	Cytokeratin 19
	Human	Purified	> 95 % determined by SDS gelelectrophoresis	E. coli	0.25 mg / €390.00
					OriGene Technologies GmbH
BA1016S	Cytokeratin 19
	Human	Purified	> 95 % determined by SDS gelelectrophoresis	E. coli	0.1 mg / €270.00
					OriGene Technologies GmbH
PA1286	Cytokeratin 19
	Human	Purified	> 95 % > 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained	E. coli
					OriGene Technologies GmbH
PA1286X	Cytokeratin 19
	Human	Purified	> 95 % > 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained	E. coli
					OriGene Technologies GmbH
PA1286XC	Cytokeratin 19
	Human	Purified	> 95 % > 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained	E. coli
					OriGene Technologies GmbH
H00003880-P01-10	Cytokeratin 19
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00003880-P01-25	Cytokeratin 19
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 19 (4 products)

Catalog No.	Species
H00003880-T01	Cytokeratin-19 (KRT19) Lysate(Denatured)

		Abnova Taiwan Corp.
H00003880-T02	Cytokeratin-19 (KRT19) Lysate(Denatured)

		Abnova Taiwan Corp.
NBL1-12387	Cytokeratin 19 Lysate

		Novus Biologicals Inc.
LY419428	KRT19 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Recommended Secondary Antibodies for Cytokeratin 19 (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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DM188 Cytokeratin 19 antibody

Quick Overview

Product Description for Cytokeratin 19

Properties for Cytokeratin 19

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 19 (12 products)

Positive controls for Cytokeratin 19 (4 products)

Recommended Secondary Antibodies for Cytokeratin 19 (11 products)

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