AP21498AF-N Bovine IgM (Fc specific) antibody

Search for all "Bovine IgM"

10 mg / €450.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AP21498AF-N

Product Description for Bovine IgM

Properties: (Fc specific)
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Bovine IgM

Product Category Secondary Antibodies
Quantity 10 mg
Synonyms Bovine Immunoglobulin M
Presentation Azide Free
Reactivity Bov
Applications Dot, E, ICC/IF, ID, IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgM isolated from pooled Bovine serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase Immunochemistry.
Recommneded Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized hyperimmune IgG fraction
Azide Free
Reconstitution:
Restore by adding 1.0 ml of sterile distilled water
Specificity
Specificity:
Ths antibody is directed against the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in Bovine serum, as tested by Immunoelectrophoresis and double Radial Immunodiffusion.
Cross-reactivity : Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Bovine.
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