Product Description for Rat IgG2b
Properties: (subclass specific)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Rat IgG2b
| Product Category | Secondary Antibodies |
|---|---|
| Quantity | 1 ml |
| Synonyms | Rat Immunoglobulin 2b |
| Presentation | Serum |
| Reactivity | Rt |
| Applications | ID, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application | In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2b from serum or plasma. Recommended working dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less then 1/32 when tested against pooled normal rat serum in agar block immunodiffusion titration. |
| Concentration | Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. |
| Storage | Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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