AP21427FC-N Human IgG (F(ab)2 specific) antibody

Search for all "Human IgG"

1 ml / €390.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AP21427FC-N

Product Description for Human IgG

Properties: (F(ab)2 specific)
Presentation: FITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence.

Properties for Human IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Human Immunoglobulin G
Presentation FITC
Reactivity Hu
Applications C, E, ICC/IF, ID
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80. 
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Concentration 4.7 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate
FITC
Absorption emission:
492 nm / 515 nm
Molar radio:
Fluorochrome/IgG = ~1.8
Purification:
Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2 without presrevatives.
State:
Lyophilized Fab fragments.
Reconstitution:
Restore by adding 1 ml sterile di stilled water.
Specificity
Specificity:
The antiserum is reacting with to the Fab subunits of the IgG, IgA, IgM and other Ig classes of both light chain type, with their Fab or F(ab’)2 subunits and with free immunoglobulin light chains of kappa and lambda type. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants.
Cross-reactivity of this conjugate has not been tested in detail.
Species:
Human.
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