AM20252PU-N Human IgG1 (Fc specific) antibody

Search for all "Human IgG1"

0.2 mg / €530.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AM20252PU-N

Product Description for Human IgG1

Properties: (Fc specific)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Human IgG1

Product Category Secondary Antibodies
Quantity 0.2 mg
Synonyms Human Immunoglobulin G1
Presentation Purified
Reactivity Hu
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone NI 132 (HP 6186)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Highly purified monoclonal IgG1 isolated from pooled Human serum.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Concentration 0.4 mg/ml
General Readings
  1. Jefferis R, Reimer CB, Skvaril F, de Lange GG, Goodall DM, Bentley TL, et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study. Immunol Lett. 1992 Feb;31(2):143-68. PubMed PMID: 1371266.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Buffer System:
PBS, pH 7.2 without preservatives or foreign proteins
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Restore by adding 0.5 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG1 molecule as tested in indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and indirect Immunoperoxidase staining of cytoplasmic Ig.
Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Species:
Human.
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