AM20254HR-N Human IgG2 (Fc specific) antibody

Search for all "Human IgG2"

0.2 mg / €650.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AM20254HR-N

Product Description for Human IgG2

Properties: (Fc specific)
Presentation: HRP
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Human IgG2

Product Category Secondary Antibodies
Quantity 0.2 mg
Synonyms Human Immunoglobulin G2
Presentation HRP
Reactivity Hu
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone NI 25-1 (HP 6207)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Highly purified monoclonal IgG2 isolated from pooled Human serum.
Application To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/100 upwards.
Western blot: from 1/250 upwards.
Concentration 0.4 mg/ml
General Readings
  1. Jefferis R, Reimer CB, Skvaril F, de Lange GG, Goodall DM, Bentley TL, et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study. Immunol Lett. 1992 Feb;31(2):143-68. PubMed PMID: 1371266.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Molar radio:
Peroxidase/IgG ~1.7
Buffer System:
PBS, pH 7.2 without preservatives or foreign proteins
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore by adding 0.5 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Species:
Human.
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