AM20255BT-N Human IgG3 (F(ab)2 specific) antibody

Search for all "Human IgG3"

0.2 mg / €650.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AM20255BT-N

Product Description for Human IgG3

Properties: (F(ab)2 specific)
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Human IgG3

Product Category Secondary Antibodies
Quantity 0.2 mg
Synonyms Human Immunoglobulin G3
Presentation Biotin
Reactivity Hu
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone NI 330 (HP 6050)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Highly purified monoclonal IgG3 isolated from pooled Human serum.
Isotype control SM10B (for use in human samples)
Add. information Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.
Application To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250. 
ELISA: from 1/1,000 upwards. 
Western blot: from 1/500 upwards.
Concentration 0.4 mg/ml
General Readings
  1. Jefferis R, Reimer CB, Skvaril F, de Lange GG, Goodall DM, Bentley TL, et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study. Immunol Lett. 1992 Feb;31(2):143-68. PubMed PMID: 1371266.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Biotin
Molar radio:
Biotin/IgG ~2.2
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore by adding 0.5 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, Immunoblotting, Immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
Cross-Reactivity: The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Species:
Human.
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