AM06072PU-N Human IgG antibody

Search for all "Human IgG"

0.1 ml / €240.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Product Description for Human IgG

Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Human IgG

Product Category Secondary Antibodies
Quantity 0.1 ml
Synonyms Human Immunoglobulin G
Presentation Purified
Reactivity Hu
Applications E, WB
Clonality Monoclonal
Clone 4D2D9G8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human IgG was isolated from Human sera and purified by chromatography.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
General Readings
  1. Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition.
  2. Vlug, A. et al "The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.03% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
Monoclonal anti-Human IgG is derived from the hybridoma 1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole Human IgG molecule, has no across action with Human IgM molecule as determined by an ELISA.
Reactivity is observed with all Human IgG subclasses but not with the Fab fragment of Human IgG, the antibody site is located in the terminal end of Human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
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