AP31448HR-N Monkey IgG (H+L chain) antibody

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1 ml / €280.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AP31448HR-N

Product Description for Monkey IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Monkey IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Monkey Immunoglobulin G
Presentation HRP
Reactivity Mky
Applications C, Dot, E, ICC/IF, ID, IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgG isolated from pooled Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Concentration 10.0 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
HRP
Molar radio:
~1.7
Purification:
DEAE-Column Chromatography
Buffer System:
PBS, pH 7.2
No preservative added, as it may interfere with the antibody activity.
No foreign proteins added.
State:
Lyophilized hyperimmune IgG fraction
Reconstitution:
Restore with 1.0 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Monkey.
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