AP31541SU-N Canine IgG (H+L chain) antibody

Search for all "Canine IgG"

1 ml / €260.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AP31541SU-N

Product Description for Canine IgG

Properties: (H+L chain)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Canine IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms dog IgG, dog immunoglobulin G
Presentation Serum
Reactivity Can
Applications ID, IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal dog serum; to prepare an immunoadsorbent for the production of Ig-free dog serum, plasma or non-Ig preparations.
Directions for use:
In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
No preservative added, as it may interfere with the antibody activity. No foreign protein added.
State:
Delipidated, heat inactivated lyophilized stable whole serum
Serum
Reconstitution:
Restore with 1 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Canine.
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