Porcine IgG (H+L chain) - AP33087AF-N | acris-antibodies.com

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Home » Porcine IgG

Product Description for Porcine IgG

Properties: (H+L chain)
Presentation: Azide Free
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Dot blot, Enzyme Immunoassay.

Properties for Porcine IgG

Product Category	Secondary Antibodies
Quantity	10 mg
Synonyms	Pig IgG, Pig Immunoglobulin G
Presentation	Azide Free
Reactivity	Por
Applications	Dot, E, ICC/IF, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information	Adsorption: Not required.
Application	ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration.
Concentration	10.0 mg/ml
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography. Buffer System: PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. Stabilizers: None State: Lyophilized purified hyperimmune IgG fraction Azide Free Reconstitution: Restore with 1 ml sterile distilled water.
Specificity	Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in swine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine: +, Cat: +, Chicken: -, Dog: +, Duck: -, Goat: +, Guinea pig: +, Human: +, Horse: +, Mouse: +, Monkey: +, Pigeon: -, Rat: +, Sheep: +, Turkey: -. Species: Pig.

Service

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D-32052 Herford
Germany
phone: +49-5221-34606-0
free call: 0800-227 47 46 (Germany only)
fax: +49-5221-34606-11
email: info-de@origene.com
orders: ordering-de@origene.com

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