AP31448FC-N Monkey IgG (H+L chain) antibody

Search for all "Monkey IgG"

2 ml / €460.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

AP31448FC-N

Product Description for Monkey IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Monkey IgG

Product Category Secondary Antibodies
Quantity 2 ml
Synonyms Monkey Immunoglobulin G
Presentation FITC
Reactivity Mky
Applications C, E, ICC/IF, IP
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Concentration 10 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate isomer 1 Conjugation procedure: A proprietary technique for the binding to is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
FITC
Absorption emission:
492nm/515nm
Molar radio:
1,4
Purification:
DEAE-column Chromatography
Buffer System:
PBS, pH 7.2
No preservative added. No foreign proteins added.
State:
Lyophilised hyperimmune Ig fraction
Reconstitution:
Restore with 2 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for chimpanzee and man.
Species:
Monkey.
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