SM3061P Human IgE antibody

Search for all "Human IgE"

0.1 mg / €180.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

SM3061P

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  • SM3061P
  • SM3061P

Product Description for Human IgE

Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.

Properties for Human IgE

Product Category Secondary Antibodies
Quantity 0.1 mg
Synonyms Immunoglobulin E human
Presentation Purified
Reactivity Hu
Applications E, F
Clonality Monoclonal
Clone BE5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified human IgE
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA.
Flow Cytometry: 5-10 µg/ml.
Background Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Kaulfürst-Soboll H, Mertens M, Brehler R, von Schaewen A. Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis. PLoS One. 2011 Mar 14;6(3):e17800. doi: 10.1371/journal.pone.0017800. PubMed PMID: 21423762. (Free PMC Article available, 7 images available)
General Readings
  1. Franklin EC. Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. PubMed PMID: 47690.
  2. Fuller JM, Keyser JW. Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71. PubMed PMID: 1122610.
  3. Balogh Z, Merétey K, Falus A, Bozsóky S. Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34. PubMed PMID: 6966908. (Free PMC Article available)
  4. Brinkmann V, Heusser CH. T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32. PubMed PMID: 8258141.
  5. Gould HJ, Beavil RL, Vercelli D. IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation. Br Med Bull. 2000;56(4):908-24. PubMed PMID: 11359628.
  6. Kaulfürst-Soboll H, Mertens M, Brehler R, von Schaewen A. Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis. PLoS One. 2011 Mar 14;6(3):e17800. doi: 10.1371/journal.pone.0017800. PubMed PMID: 21423762. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Precipitation methods and ion exchange chromatography; purity: > 95% (by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State:
Liquid Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The antibody reacts with human IgE; it recognizes an epitope different from the ones recognized by clone 4G7 and clone 4H10 antibodies to IgE.
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