SM3063APC Human IgM (Fc-region) antibody

Search for all "Human IgM"

0.1 mg / €270.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

SM3063APC

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Product Description for Human IgM

Properties: (Fc-region)
Presentation: APC
Product is tested for Flow Cytometry.

Properties for Human IgM

Product Category Secondary Antibodies
Quantity 0.1 mg
Synonyms Human Immunoglobulin M
Presentation APC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone CH2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified Human IgM
Isotype control SM10APC (for use in human samples)
Application Flow Cytometry: 2 µg/ml is recommended starting point.
Background Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Concentration 0.1 mg/ml
General Readings
  1. Franklin EC. Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. PubMed PMID: 47690.
  2. Fuller JM, Keyser JW. Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71. PubMed PMID: 1122610.
  3. Balogh Z, Merétey K, Falus A, Bozsóky S. Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34. PubMed PMID: 6966908. (Free PMC Article available)
  4. Brinkmann V, Heusser CH. T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32. PubMed PMID: 8258141.
Storage Store the antibody undiluted at 2-8 °C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Should it contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Format
Label:
Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
APC
Buffer System:
Phosphate buffered saline (PBS) solution containing 15mM sodium azide
State:
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The antibody (Clone CH2) reacts with Fc fragment of Human IgM.
Specificity was confirmed by Western blotting analysis of purified Human IgM under reducing conditions and also by Immunofluorescence staining of Daudi cells (the antibody CH2 blocks binding of standard antibody to Fc fragment of Human IgM and does not block binding of standard antibody to Fab fragment of Human IgM).
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