AR51993PU-S PGD / PGDH (1-483, His-tag)

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20 µg / €180.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

PGD / PGDH

Product Description for PGD / PGDH

PGD / PGDH.
Presentation: Purified

Properties for PGD / PGDH

Product Category Proteins & Growth Factors
Quantity 20 µg
Synonyms 6-phosphogluconate dehydrogenase decarboxylating
Presentation Purified
Molecular weight 55.3 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P52209
Purity > 90 % by SDS - PAGE
Background PGD (Phosphoglucote dehydroges), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of DPH. PGD deficiency increases the level of erythrocyte pyruvate kise (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal domint fashion. Recombint human PGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventiol chromatography techniques.
Concentration 1.0 mg/ml (determined by bradford assay)
General Readings Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107(7):3198-203.Tagen M., et al. (2009) J Immunol. 183(10):6313-9.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA
Molecular weight:
(503aa)
Specific activity:
Specific activity is > 10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of ß-NADP.
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol.
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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