AR51996PU-N Carbonic anhydrase 1 (1-261, His-tag)

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0.1 mg / €400.00
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Carbonic anhydrase 1

Product Description for Carbonic anhydrase 1

Carbonic anhydrase 1.
Presentation: Purified

Properties for Carbonic anhydrase 1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms CA-I, CA1, CAB, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I
Presentation Purified
Molecular weight 31.0 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00915
Purity > 95 % by SDS - PAGE
Background Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbote and protons (CO2 + H2O <-> HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombint carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventiol chromatography techniques.
Concentration 1.0 mg/ml (determined by bradford assay)
General Readings Nogradi A., et al. (1998). Am J Pathol. 153:1-4.Ferry JG., et al. (1999). Proc Natl Acad Sci U S A. 96(26):15184-9.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
Molecular weight:
(281aa), confirmed by MALDI-TOF
Specific activity:
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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