discontinued

TA326519 CACNG2 (203-323, Cytopl. Dom.) antibody

Mouse Monoclonal Anti-TARPGamma2/Stargazin Antibody

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Mouse anti Mouse, Rat CACNG2 S245-1

Product Description for CACNG2

Mouse anti Mouse, Rat CACNG2 S245-1.
Properties: (203-323, Cytopl. Dom.)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for CACNG2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Neuronal voltage-gated calcium channel gamma-2 subunit, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Voltage-dependent calcium channel gamma-2 subunit
Presentation Purified
Reactivity Ms, Rt
Applications ICC/IF, WB
Clonality Monoclonal
Clone S245-1
Host Mouse
Isotype IgG2a
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q71RJ2
Immunogen:
Fusion protein amino acids 203-323 (cytoplasmic, C-terminus) of rat TARP Gamma2 / CACNG2
Property 203-323, Cytopl. Dom.
Isotype control AM03096PU-N
Application Immunocytochemistry.
Western Blot:
 1 μg/ml of this antibody was sufficient for detection of CACNG2 in 20μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG-HRP as the secondary antibody.
Background
Calcium channel, voltage-dependent, gamma subunit 2, also known as CACNG2 or stargazin is a protein that in humans is encoded by the CACNG2 gene. CACNG2 protein (Stargazin)  is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
Concentration 1.0 mg/ml
General Readings
  1. Gill MB, Kato AS, Roberts MF, Yu H, Wang H, Tomita S, et al. Cornichon-2 modulates AMPA receptor-transmembrane AMPA receptor regulatory protein assembly to dictate gating and pharmacology. J Neurosci. 2011 May 4;31(18):6928-38. doi: 10.1523/JNEUROSCI.6271-10.2011. PubMed PMID: 21543622.
  2. Yu L, Rostamiani K, Hsu YT, Wang Y, Bi X, Baudry M. Calpain-mediated regulation of stargazin in adult rat brain. Neuroscience. 2011 Mar 31;178:13-20. doi: 10.1016/j.neuroscience.2011.01.026. Epub 2011 Jan 20. PubMed PMID: 21256931. (Free PMC Article available, 7 images available)
  3. Steinmetz CC, Turrigiano GG. Tumor necrosis factor-α signaling maintains the ability of cortical synapses to express synaptic scaling. J Neurosci. 2010 Nov 3;30(44):14685-90. doi: 10.1523/JNEUROSCI.2210-10.2010. PubMed PMID: 21048125. (Free PMC Article available, 3 images available)
  4. Kato AS, Zhou W, Milstein AD, Knierman MD, Siuda ER, Dotzlaf JE, et al. New transmembrane AMPA receptor regulatory protein isoform, gamma-7, differentially regulates AMPA receptors. J Neurosci. 2007 May 2;27(18):4969-77. PubMed PMID: 17475805.
  5. Letts VA, Felix R, Biddlecome GH, Arikkath J, Mahaffey CL, Valenzuela A, et al. The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit. Nat Genet. 1998 Aug;19(4):340-7. PubMed PMID: 9697694.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Protein G chromatography
Buffer System:
PBS pH 7.4, 50% Glycerol
Preservatives:
0.09% Sodium azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody detects  CACNG2; ~ 36 kDa.
Does not cross-react with CACNG3, CACNG4 or CACNG8.
Species:
Mouse, Rat.

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