Product Description for POLE3
Rabbit anti Bovine, Canine, Human, Mouse, Rat, Zebrafish, African clawed frog POLE3.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties for POLE3
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | Arsenic-transactivated protein, CHRAC17, Chromatin accessibility complex 17, DNA polymerase II subunit 3, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17 |
| Presentation | Aff - Purified |
| Reactivity | African clawed frog, Bov, Can, Hu, Ms, Rt, Ze |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Molecular weight | 17 kDa (147 amino acids) |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western blotting (2-5 µg/ml). |
| Background | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. |
| General Readings | Bolognese,F., et al., (2000) Nucleic Acids Res. 28 (19), 3830-3838 |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for POLE3 (7 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP300874 |
POLE3 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR50965PU-N |
POLE3 (1-147, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR50965PU-S |
POLE3 (1-147, His-tag) |
||||||
|
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| H00054107-P01-10 |
POLE3 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00054107-P01-25 |
POLE3 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| 10-288-22369F |
POLE3 |
||||||
|
|
Human | Purified | 95 % | E. coli | |||
| GenWay Biotech Inc. | |||||||
| 10-288-22369F-50 |
POLE3 |
||||||
|
|
Human | Purified | 95 % | E. coli | |||
| GenWay Biotech Inc. | |||||||
Positive controls for POLE3 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00054107-T01 |
POLE3 293T Cell Transient Overexpression Lysate(Denatured) |
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|
|||||||
| Abnova Taiwan Corp. | |||||||
| H00054107-T02 |
POLE3 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for POLE3 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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