Product Description for TRIM13 / RNF77
Rabbit anti Bovine, Human, Mouse, Rat TRIM13 / RNF77.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Properties for TRIM13 / RNF77
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | B-cell chronic lymphocytic leukemia tumor suppressor Leu5, LEU5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RFP2, RING finger protein 77, Tripartite motif-containing protein 13 |
| Presentation | Aff - Purified |
| Reactivity | Bov, Hu, Ms, Rt |
| Applications | P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
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|---|---|
| Application | Western blotting (0.1 - 0.5 µg/ml) Immunohistochemsitry on paraffin embedded sections (2 - 8 µg/ml) |
| Background | TRIM13 is an E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. TRIM13 enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation, regulates ER stress-induced autophagy, and may act as a tumor suppressor. |
| General Readings | "The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD." Lerner M., Corcoran M., Cepeda D., Nielsen M.L., Zubarev R., Ponten F., Uhlen M., Hober S., Grander D., Sangfelt O. Mol. Biol. Cell 18:1670-1682(2007) "Ret finger protein 2 enhances ionizing radiation-induced apoptosis via degradation of AKT and MDM2." Joo H.M., Kim J.Y., Jeong J.B., Seong K.M., Nam S.Y., Yang K.H., Kim C.S., Kim H.S., Jeong M., An S., Jin Y.W. Eur. J. Cell Biol. 90:420-431(2011) "TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells." Tomar D., Singh R., Singh A.K., Pandya C.D., Singh R. Biochim. Biophys. Acta 1823:316-326(2012) |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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| Species Reactivity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for TRIM13 / RNF77 (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP309480 |
TRIM13 / RNF77 (transcript variant 3) |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00010206-P01-10 |
TRIM13 / RNF77 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010206-P01-25 |
TRIM13 / RNF77 |
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|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010206-Q01-10 |
TRIM13 / RNF77 |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00010206-Q01-25 |
TRIM13 / RNF77 |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for TRIM13 / RNF77 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-17276 |
TRIM13 Lysate |
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![Western Blot: TRIM13 Lysate [NBL1-17276] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for TRIM13](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/TRIM13%20Lysate-Western%20Blot-NBL1-17276-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for TRIM13 / RNF77 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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