Product Description for ABC2 / SMG8
Rabbit anti Human ABC2 / SMG8.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties for ABC2 / SMG8
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | Amplified in breast cancer gene 2 protein, UPF0487 protein C17orf71 |
| Presentation | Aff - Purified |
| Reactivity | Hu |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western blotting (0.2 -1 µg/ml). |
| Background | ABC2 or SMG8 is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. It ss recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). SMG8 acts as a regulator of kinase activity. |
| General Readings | "SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay." Yamashita A., Izumi N., Kashima I., Ohnishi T., Saari B., Katsuhata Y., Muramatsu R., Morita T., Iwamatsu A., Hachiya T., Kurata R., Hirano H., Anderson P., Ohno S. Genes Dev. 23:1091-1105(2009) |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for ABC2 / SMG8 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP316692 |
ABC2 / SMG8 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|||
| OriGene Technologies, Inc. | |||||||
Positive controls for ABC2 / SMG8 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY413279 |
SMG8 overexpression lysate |
||||||
 |
0.1 mg /
€495.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for ABC2 / SMG8 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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