discontinued

AM01314PU-N Epstein Barr Virus / EBV LMP2A antibody

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Rat anti Epstein Barr Virus / EBV 14B7

AM01314PU-N

Product Description for Epstein Barr Virus / EBV

Rat anti Epstein Barr Virus / EBV 14B7.
Properties: LMP2A
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Epstein Barr Virus / EBV

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms HHV-4, HHV4
Presentation Purified
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone 14B7
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Bacterial TrpE-LMP2A fusion protein. Spleen cells from immunised Lou/C rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
Property LMP2A
Isotype control SM15P, SM15PX
Application Immunoprecipitation.
Immunofluorescence.
Western Blot: 1/100 - 1/1000.
Not suitable for paraffin sections.
Background EBV is a human herpesvirus, which is associated with conditions such as Hodgkins disease and Burkitts Lymphoma and is the causative agent in mononucleosis in adolescents. EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals. Clone 14B7 detects wild-type LMP2A protein.
Concentration 1.0 mg/ml
General Readings
  1. Fruehling S, Lee SK, Herrold R, Frech B, Laux G, Kremmer E, et al. Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction. J Virol. 1996 Sep;70(9):6216-26. PubMed PMID: 8709248. (Free PMC Article available)
  2. Fruehling S, Swart R, Dolwick KM, Kremmer E, Longnecker R. Tyrosine 112 of latent membrane protein 2A is essential for protein tyrosine kinase loading and regulation of Epstein-Barr virus latency. J Virol. 1998 Oct;72(10):7796-806. PubMed PMID: 9733815. (Free PMC Article available, 9 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide
State:
Liquid purified Ig
Purified
Specificity
Specificity:
This antibody recognises latent membrane protein 2A (LMP2A) of Epstein-Barr virus (EBV).

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