AP02276PU-S Collagen type VII antibody

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10 µg / €190.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse Collagen type VII

AP02276PU-S

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  • AP02276PU-S

Product Description for Collagen type VII

Rabbit anti Human, Mouse Collagen type VII.
Presentation: Ig Fraction
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Collagen type VII

Product Category Primary Antibodies
Quantity 10 µg
Synonyms COL7A1, LC collagen, Long-chain collagen
Presentation Ig Fraction
Reactivity Hu, Ms
Applications C, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q63870
Immunogen:
Recombinant Murine type VII Collagen (mixture of fragments aa 97-200, aa 479-578, aa 757-967) GST fusion protein­.
GeneID:
12836
Application ELISA: 1/5,000. 
Western blot: 1/500 (Ref.1).
Immunofluorescence on Frozen Sections: 1/100 (Ref.1)
Background Type VII Collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.
General Readings
  1. Sitaru C, Mihai S, Otto C, Chiriac MT, Hausser I, Dotterweich B, et al. Induction of dermal-epidermal separation in mice by passive transfer of antibodies specific to type VII collagen. J Clin Invest. 2005 Apr;115(4):870-8. PubMed PMID: 15841176. (Free PMC Article available, 9 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS
State:
Lyophilized purified IgG fraction from Serum
Ig Fraction
Reconstitution:
Restore in aqua bidest to a concentration of 1 mg/ml.
Specificity
Specificity:
This antibody detects Recombinant Murine type VII Collagen (aa 757-967).
Cross reacts with Human type VII Collagen.
Not tested for reactivity with type VII Collagen from other species.
Species:
Human, Mouse.
Gene ID 12836

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