Apolipoprotein A I / APO AI - BM2577

Product Description for Apolipoprotein A I / APO AI

Mouse anti Human Apolipoprotein A I / APO AI 1405.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Radioimmunoassay.

Properties for Apolipoprotein A I / APO AI

Product Category	Primary Antibodies
Quantity	1 mg
Synonyms	APOA1, Apo-AI, ApoA-I, ApoAI
Presentation	Purified
Reactivity	Hu
Applications	E, R
Clonality	Monoclonal
Clone	1405
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P02647 Immunogen: Purified Human plasma Apo A-I GeneID: 335
Isotype control	AM03095PU-N, SM10P (for use in human samples)
Application	Suitable for use in Competitive RIA or EIA.
Background	Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
Concentration	3.47 mg/ml (OD280 nm, E1% = 14)
General Readings	Jamil H, Gordon DA, Eustice DC, Brooks CM, Dickson JK, Chen Y, et al. An inhibitor of the microsomal triglyceride transfer protein inhibits apoB secretion from HepG2 cells. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11991-5. PubMed PMID: 8876250. (Free PMC Article available) Ishiguro H, Yoshida H, Major AS, Zhu T, Babaev VR, Linton MF, et al. Retrovirus-mediated expression of apolipoprotein A-I in the macrophage protects against atherosclerosis in vivo. J Biol Chem. 2001 Sep 28;276(39):36742-8. Epub 2001 Jul 26. PubMed PMID: 11477092.
Storage	Store the antibody undiluted at 2-8°C for one mounth or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Protein A Chromatography. Buffer System: PBS, pH 7.0 containing 0.02% Sodium Azide as preservative. State: Liquid purified IgG fraction. Purified
Specificity	Specificity: Recognizes Apolipoprotein A1 (Apo AI). No cross reactivity with Apo A-II or Apo B. Aff. Const.: 8 x 10e9 Species: Human.
Gene ID	335

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein A I / APO AI (17 products)

Catalog No.	Species	Pres.	Purity	Source
TP310762	Apolipoprotein A I / APO AI
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
TP720424	Apolipoprotein A I / APO AI
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	10 µg / €299.00
					OriGene Technologies, Inc.
TP723014	Apolipoprotein A I / APO AI
	Human		> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	20 µg / €135.00
					OriGene Technologies, Inc.
AR00018PU-N	Apolipoprotein A I / APO AI
	Hamster	Purified	Syrian hamster plasma, high density lipoproteins	Plasma	0.1 mg / €1,930.00
					OriGene Technologies GmbH
AR09244PU-L	Apolipoprotein A I / APO AI (25-267, His-tag)
	Human	Purified	> 95 % by SDS - PAGE	E. coli	0.5 mg / €820.00
					OriGene Technologies GmbH
AR09244PU-N	Apolipoprotein A I / APO AI (25-267, His-tag)
	Human	Purified	> 95 % by SDS - PAGE	E. coli	0.1 mg / €320.00
					OriGene Technologies GmbH
AR09503PU-N	Apolipoprotein A I / APO AI
	Human		> 97 % pure by SDS-PAGE gel and HPLC analyses.	E. coli
					OriGene Technologies GmbH
AR09503PU-S	Apolipoprotein A I / APO AI
	Human		> 97 % pure by SDS-PAGE gel and HPLC analyses.	E. coli
					OriGene Technologies GmbH
AR31065PU-N	Apolipoprotein A I / APO AI
	Human	Purified	> 90 %	Plasma	0.5 mg / €340.00
					OriGene Technologies GmbH
BA051	Apolipoprotein A I / APO AI
	Human	Purified	> 99 % pure by SDS-PAGE. Purification: The protein was isolated by ultracentrifugal flotation between densities 1.063-1.21 g/ml, lyophilized then subjected to repeated Ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6 M Guanidine Hydrochloride -25 mM DTT. The product was then subjected to Sephacryl S200 filtration and eluted.	Plasma	1 mg / €390.00
					OriGene Technologies GmbH
BA681	Apolipoprotein A I / APO AI
	Human	Purified	> 95 % pure by SDS-PAGE (Column Chromatography).	Plasma	0.5 mg / €260.00
					OriGene Technologies GmbH
BA1021	Apolipoprotein A I / APO AI
	Mouse	Purified	Purified from Swiss-Webster Mouse high density lipoprotein plasma fraction by gel-sieving- and Ion Exchange Column Chromatography	Plasma
					OriGene Technologies GmbH
H00000335-P01-10	Apolipoprotein A I / APO AI
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00000335-P01-25	Apolipoprotein A I / APO AI
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P5326	Apolipoprotein A I / APO AI
	Human
					Abnova Taiwan Corp.
P5942	Apolipoprotein A I / APO AI
	Human
					Abnova Taiwan Corp.
NBP1-30197	Apolipoprotein A I / APO AI

					Novus Biologicals Inc.

Positive controls for Apolipoprotein A I / APO AI (2 products)

Catalog No.	Species	Purity
H00000335-T01	Apolipoprotein A I (APO AI) Lysate(Denatured)

			Abnova Taiwan Corp.
NBL1-07609	Apolipoprotein A I Lysate
		Protein
			Novus Biologicals Inc.

Recommended Secondary Antibodies for Apolipoprotein A I / APO AI (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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BM2577 Apolipoprotein A I / APO AI antibody

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Product Description for Apolipoprotein A I / APO AI

Properties for Apolipoprotein A I / APO AI

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein A I / APO AI (17 products)

Positive controls for Apolipoprotein A I / APO AI (2 products)

Recommended Secondary Antibodies for Apolipoprotein A I / APO AI (11 products)

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