BP1076B Vaccinia Virus (Lister Strain) antibody

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1 ml / €490.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Vaccinia Virus

BP1076B

Product Description for Vaccinia Virus

Rabbit anti Vaccinia Virus.
Properties: (Lister Strain)
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Paraffin Sections.

Properties for Vaccinia Virus

Product Category Primary Antibodies
Quantity 1 ml
Presentation Biotin
Applications E, P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Lister Strain (mixture of virions and ICPs)
Property Lister Strain
Application Avidin and streptavidin amplification systems for ELISA and Immunohistochemistry
Protein K digestion is recommended with Formalin-Fixed Paraffin Embedded Sections. 
May be used in place of neat antiserum in almost any appropriate antibody-based technique.
Background Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
Concentration 4-5 mg/ml (OD280 nm, E0.1% = 1.4)
General Readings
  1. Fedorko DP, Preuss JC, Fahle GA, Li L, Fischer SH, Hohman P, et al. Comparison of methods for detection of vaccinia virus in patient specimens. J Clin Microbiol. 2005 Sep;43(9):4602-6. PubMed PMID: 16145113. (Free PMC Article available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Covalently coupled with the N-Hydroxysuccinimide ester of under mild conditions to give a high degree of substitution
Biotin
Purification:
Protein A Chromatography
Buffer System:
0.01M PBS pH 7.2 containing 0.09% Sodium Azide as preservative
Contains no stabilizing proteins.
State:
Liquid purified Ig fraction (>95% pure)
Specificity
Specificity:
Recognizes purified virions.
Does not cross-react with Parainfluenza (1-3), RSV, Adenovirus, Influenza A & B or HSV-1.
Does not react with uninfected cells.
Reactive with Lister, Wyeth, New York City and MVA strains of Vaccinia.

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