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BP2096 Complement factor H (CFH) (Beta 1H) antibody

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Quick Overview

Sheep anti Canine, Equine, Feline, Guinea Pig, Human, Mouse, Rat Complement factor H (CFH)

BP2096

Product Description for Complement factor H (CFH)

Sheep anti Canine, Equine, Feline, Guinea Pig, Human, Mouse, Rat Complement factor H (CFH).
Properties: (Beta 1H)
Presentation: Ig Fraction
Product is tested for Enzyme Immunoassay, Immunodiffusion.

Properties for Complement factor H (CFH)

Product Category Primary Antibodies
Quantity 1 ml
Synonyms CFH, H factor 1, HF, HF1, HF2
Presentation Ig Fraction
Reactivity Can, Eq, Fe, GP, Hu, Ms, Rt
Applications E, ID
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08603
Immunogen:
Human 155 kDa Factor H purified from plasma, shown to be pure by IEP.
GeneID:
3075
Property Beta 1H
Application Suitable for use in a variety of Gel techniques including Radial Immunodiffusion (RID), Double Diffusion and Immunoelectrophoresis (IEP).
RID: 1 µl antiserum/cm2 gel vs. 5 µl neat-1/10 dilution Human plasma.
Double Diffusion: 10 µl antiserum vs. 10 µl plasma.
IEP: 100 µl antiserum vs. 5 µl plasma.
The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or tris-barbital pH > 8.2) is recommended.
Background The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy.
Concentration 19.3 mg/ml (OD280 nm, E1% = 14.5)
General Readings
  1. Chung KM, Liszewski MK, Nybakken G, Davis AE, Townsend RR, Fremont DH, et al. West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H. Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):19111-6. Epub 2006 Nov 28. PubMed PMID: 17132743. (Free PMC Article available, 5 images available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Slight precipitation can occur on storage which may be removed by centrifugation and should not affect performance characteristics.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Delipidation and Absorbtion to monospecificity by use of solid-phase absorbants. The titer is adjusted so that inter-batch variation is within 10%. Product is 0.2 µm filtered.
Buffer System:
Glycine buffered saline, pH 7.4 with 0.09% Sodium Azide, 0.1% EACA, 0.01% Benzamidine and 1 mM EDTA.
State:
Liquid purified Ig fraction
Ig Fraction
Species Reactivity
Species reactivity (tested):
Cat + Chicken - Cow - Dog + Guinea Pig + Horse + Human + Mouse + Pig - Rabbit - Rat + Goat -
Specificity
Specificity:
Gives a single arc when tested by IEP against Human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs. Human plasma and a known anti-Human Factor H.
The cross reactivity of this product against the sera of the species listed below has been assessed by Double Diffusion using 10 µl of antisera against 10 µl of species sera. These results are intended as a guideline only.
It is possible that cross-reactivity against species listed as negative above may be observed when using different test conditions or techniques.
Gene ID 3075

Accessory Products

Proteins and/or Positive Controls

Proteins for Complement factor H (CFH) (8 products)

Catalog No. Species Pres. Purity   Source  

Complement factor H (CFH)
Complement factor H (CFH) Human > 95 % pure by SDS-PAGE. Serum
  OriGene Technologies GmbH

Complement factor H (CFH)
Complement factor H (CFH) Human
  Abnova Taiwan Corp.

Complement factor H (CFH)
Complement factor H (CFH) Human Purified
  Abnova Taiwan Corp.

Complement factor H (CFH)
Complement factor H (CFH) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Complement factor H (CFH)
Complement factor H (CFH) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Complement factor H (CFH)
Complement factor H (CFH) Human Purified
  Abnova Taiwan Corp.

Complement factor H (CFH)
Complement factor H (CFH) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Complement factor H (CFH)
Complement factor H (CFH) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for Complement factor H (CFH) (2 products)

Catalog No. Species Pres. Purity   Source  

Complement factor H (CFH) Lysate(Denatured)
Complement factor H (CFH) Lysate(Denatured)
  Abnova Taiwan Corp.

Factor H Lysate
Western Blot: Factor H Lysate [NBL1-09121] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CFH
  Novus Biologicals Inc.

Recommended Secondary Antibodies for Complement factor H (CFH) (6 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment antibody
Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment antibody Donkey PE
    OriGene Technologies GmbH

Sheep IgG (H+L chain) antibody
Sheep IgG (H+L chain) antibody Rabbit AP
    OriGene Technologies GmbH

Sheep IgG (H+L chain) antibody
Sheep IgG (H+L chain) antibody Rabbit Biotin
    OriGene Technologies GmbH

Sheep IgG (H+L chain) antibody
Sheep IgG (H+L chain) antibody Rabbit FITC
    OriGene Technologies GmbH

Sheep IgG (H+L chain) antibody
Sheep IgG (H+L chain) antibody Rabbit HRP
    OriGene Technologies GmbH

Sheep IgG (H+L chain) antibody
Sheep IgG (H+L chain) antibody Rabbit TRITC
    OriGene Technologies GmbH
Click here to see all secondary antibodies for Complement factor H (CFH)
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