BP464HRP FITC antibody

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1 ml / €530.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti FITC

BP464HRP

Product Description for FITC

Rabbit anti FITC.
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for FITC

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Fluorescein Isothiocyanate
Presentation HRP
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
FITC-KLH conjugate
Application Suitable for use in Western Blot and ELISA.
Recommended Chromogens:
Diaminobenzidine or TMB for immunohistochemistry;
4-chloro napthol for Immunoblotting and
0-phenylenediamine or ABTS for Enzymeimmunoassay.
Background FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Caution Sodium Azide is a potent inhibitor of Peroxidase!
Concentration 1-2 mg/ml (OD280)
Storage Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
The affinity purified and absorbed antibody was covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed
HRP
Molar radio:
HRP:IgG is >1.5
Purification:
Solid Phase hapten-antibodies capture.
Buffer System:
Saline Phosphate containing 0.02% Thimerosal as preservative and 10 mg/ml BSA as stabilizer.
State:
Liquid purified IgG fraction.
Specificity
Specificity:
Recognizes Fluorescein.
No cross reactivity with Immunoglobulin of any species.

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