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BM6003P Cytokeratin 7 antibody

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0.1 mg / €370.00
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Mouse anti Canine, Feline, Goat, Hamster, Human, Mouse, Rat, Zebrafish Cytokeratin 7 RCK105

BM6003P

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  • BM6003P

Product Description for Cytokeratin 7

Mouse anti Canine, Feline, Goat, Hamster, Human, Mouse, Rat, Zebrafish Cytokeratin 7 RCK105.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Cytokeratin 7

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
Presentation Purified
Reactivity Can, Fe, Gt, Hst, Hu, Ms, Rt, Ze
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone RCK105
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08729
Immunogen:
Cytokeratins from the Human bladder carcinoma cell line T24.
GeneID:
3855
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunoblotting. 
Flow Cytometry.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections. 
Recommended Dilutions: 1/100-1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting .
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration 1.0 mg/ml
General Readings
  1. Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P. Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res. 1987 May;170(1):235-49. PubMed PMID: 2436934.
  2. Smedts F, Ramaekers F, Robben H, Pruszczynski M, van Muijen G, Lane B, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990 Mar;136(3):657-68. PubMed PMID: 1690513. (Free PMC Article available)
  3. Schaafsma HE, Ramaekers FC, van Muijen GN, Lane EB, Leigh IM, Robben H, et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol. 1990 Feb;136(2):329-43. PubMed PMID: 1689541. (Free PMC Article available)
  4. Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641-55. PubMed PMID: 1690512. (Free PMC Article available)
  5. Bauwens LJ, De Groot JC, Ramaekers FC, Veldman JE, Huizing EH. Expression of intermediate filament proteins in the adult human vestibular labyrinth. Ann Otol Rhinol Laryngol. 1992 Jun;101(6):479-86. PubMed PMID: 1376975.
  6. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511.
  7. Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res. 1992 Mar;53(3):304-14. PubMed PMID: 1375818.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS with 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
RCK105 reacts exclusively with Cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
Species:
Canine, Feline, Goat, Hamster, Human, Mouse, Rat, Zebrafish.
Gene ID 3855

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 7 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 7
Cytokeratin 7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Cytokeratin 7
Cytokeratin 7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 7 (3 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 7 Lysate
Western Blot: Cytokeratin 7 Lysate [NBL1-12396] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT7
  Novus Biologicals Inc.

KRT7 293T Cell Transient Overexpression Lysate(Denatured)
KRT7 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

KRT7 overexpression lysate
KRT7 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

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Mouse IgG (H+L chain), adsorbed antibody
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Click here to see all secondary antibodies for Cytokeratin 7
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