BM6004P Cytokeratin 8 antibody

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0.1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Zebrafish Cytokeratin 8 H1

Product Description for Cytokeratin 8

Mouse anti Human, Zebrafish Cytokeratin 8 H1.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Cytokeratin 8

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
Presentation Purified
Reactivity Hu, Ze
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone H1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05787
Immunogen:
Cytoskeleton preparation containing Cytokeratin 8.
GeneID:
3856
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoblotting. 
Flow Cytometry.
Immunocytochemistry.
Immunofluorescence.
Immunohistochemistry on Frozen and Paraffin-Embedded Sections.

Recommended Dilutions: 1/25-1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting applications.
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration 1.0 mg/ml
General Readings
  1. Guelstein VI, Tchypysheva TA, Ermilova VD, Litvinova LV, Troyanovsky SM, Bannikov GA. Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer. Int J Cancer. 1988 Aug 15;42(2):147-53. PubMed PMID: 2456993.
  2. Troyanovsky SM, Guelstein VI, Tchipysheva TA, Krutovskikh VA, Bannikov GA. Patterns of expression of keratin 17 in human epithelia: dependency on cell position. J Cell Sci. 1989 Jul;93 ( Pt 3):419-26. PubMed PMID: 2481679.
  3. Ljubimov AV, Bartek J, Couchman JR, Kapuller LL, Veselov VV, Kovarik J, et al. Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer. 1992 Feb 20;50(4):562-6. PubMed PMID: 1371500.
  4. Litvinov SV, van Driel W, van Rhijn CM, Bakker HA, van Krieken H, Fleuren GJ, et al. Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation. Am J Pathol. 1996 Mar;148(3):865-75. PubMed PMID: 8774141. (Free PMC Article available)
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Zebrafish.
Specificity
Specificity:
This Monoclonal antibody H1 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.
Gene ID 3856

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 8 (17 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 8

Cytokeratin 8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % determined by SDS gelelectrophoresis E. coli
0.25 mg / €520.00
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % determined by SDS gelelectrophoresis E. coli
0.1 mg / €360.00
  OriGene Technologies GmbH

Cytokeratin 8 (1-483, His-tag)

Cytokeratin 8 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

Cytokeratin 8 (1-483, His-tag)

Cytokeratin 8 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

Cytokeratin 8

Lane 1: Phosphorylase B (a), BSA (b), Ovalbumin (c), Carbonic Anhydrase (d). Lane 2: keratin K8 Bovine Purified > 95 % (determined by SDS gelelectrophoresis) Liver
0.25 mg / €390.00
  OriGene Technologies GmbH

Cytokeratin 8

Lane 1: Phosphorylase B (a), BSA (b), Ovalbumin (c), Carbonic Anhydrase (d). Lane 2: keratin K8 Bovine Purified > 95 % (determined by SDS gelelectrophoresis) Liver
0.1 mg / €270.00
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained
E. coli
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained
E. coli
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained
E. coli
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 90 % E. coli
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 90 % E. coli
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified > 90 % E. coli
  OriGene Technologies GmbH

Cytokeratin 8

Cytokeratin 8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Cytokeratin 8

Cytokeratin 8 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Cytokeratin 8

Cytokeratin 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Cytokeratin 8

Cytokeratin 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 8 (4 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin-8 (KRT8) Lysate(Denatured)

Cytokeratin-8 (KRT8) Lysate(Denatured)
  Abnova Taiwan Corp.

Cytokeratin-8 (KRT8) Lysate(Denatured)

Cytokeratin-8 (KRT8) Lysate(Denatured)
  Abnova Taiwan Corp.

Cytokeratin 8 Lysate

Western Blot: Cytokeratin 8 Lysate [NBL1-12397] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT8
  Novus Biologicals Inc.

KRT8 overexpression lysate

KRT8 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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