Product Description for Cytokeratin 8
Mouse anti Human, Zebrafish Cytokeratin 8 H1.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.
Properties for Cytokeratin 8
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8 |
| Presentation | Purified |
| Reactivity | Hu, Ze |
| Applications | C, F, ICC/IF, P, WB |
| Clonality | Monoclonal |
| Clone | H1 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | Immunoblotting. Flow Cytometry. Immunocytochemistry. Immunofluorescence. Immunohistochemistry on Frozen and Paraffin-Embedded Sections. Recommended Dilutions: 1/25-1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting applications. |
| Background | Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. |
| Concentration | 1.0 mg/ml |
| General Readings |
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| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. |
| Format |
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| Species Reactivity |
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| Specificity |
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| Gene ID | 3856 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Cytokeratin 8 (17 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP309570 |
Cytokeratin 8 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| AR09360PU-N |
Cytokeratin 8 |
||||||
|
Human | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.25 mg /
€520.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09360PU-S |
Cytokeratin 8 |
||||||
|
Human | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.1 mg /
€360.00
|
||
| OriGene Technologies GmbH | |||||||
| AR50900PU-N |
Cytokeratin 8 (1-483, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR50900PU-S |
Cytokeratin 8 (1-483, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| BA1007 |
Cytokeratin 8 |
||||||
|
Bovine | Purified | > 95 % (determined by SDS gelelectrophoresis) | Liver |
0.25 mg /
€390.00
|
||
| OriGene Technologies GmbH | |||||||
| BA1007S |
Cytokeratin 8 |
||||||
|
Bovine | Purified | > 95 % (determined by SDS gelelectrophoresis) | Liver |
0.1 mg /
€270.00
|
||
| OriGene Technologies GmbH | |||||||
| PA1283 |
Cytokeratin 8 |
||||||
|
Human | Purified |
> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1283X |
Cytokeratin 8 |
||||||
|
Human | Purified |
> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1283XC |
Cytokeratin 8 |
||||||
|
Human | Purified |
> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1362 |
Cytokeratin 8 |
||||||
|
Human | Purified | > 90 % | E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1362X |
Cytokeratin 8 |
||||||
|
Human | Purified | > 90 % | E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1362XC |
Cytokeratin 8 |
||||||
|
Human | Purified | > 90 % | E. coli | |||
| OriGene Technologies GmbH | |||||||
| H00003856-P01-10 |
Cytokeratin 8 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003856-P01-25 |
Cytokeratin 8 |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003856-Q01-10 |
Cytokeratin 8 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00003856-Q01-25 |
Cytokeratin 8 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Cytokeratin 8 (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00003856-T01 |
Cytokeratin-8 (KRT8) Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
| H00003856-T02 |
Cytokeratin-8 (KRT8) Lysate(Denatured) |
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|||||||
| Abnova Taiwan Corp. | |||||||
| NBL1-12397 |
Cytokeratin 8 Lysate |
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|||||||
| Novus Biologicals Inc. | |||||||
| LY419425 |
KRT8 overexpression lysate |
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0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for Cytokeratin 8 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: Cytokeratin 8 Lysate [NBL1-12397] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for KRT8](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Cytokeratin%208%20Lysate-Western%20Blot-NBL1-12397-img0001.jpg)
