Cytokeratin 18 - BM6007P | acris-antibodies.com

Product Description for Cytokeratin 18

Mouse anti Canine, Feline, Human, Zebrafish Cytokeratin 18 DE-K18.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Cytokeratin 18

Product Category	Primary Antibodies
Quantity	0.1 mg
Synonyms	CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Presentation	Purified
Reactivity	Can, Fe, Hu, Ze
Applications	C, F, ICC/IF, P, WB
Clonality	Monoclonal
Clone	DE-K18
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P05783 Immunogen: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431. GeneID: 3875
Isotype control	AM03095PU-N, SM10P (for use in human samples)
Application	Suitable for Immunocytochemistry, Immunoblotting (1/100-1/1000), Flow cytometry (1/100-1/200) and Immunohistochemistry on frozen (with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent) and paraffin embedded tissues (pretreatment with 0,1% pepsin in 0.1 N HCl 30 min at room temperature is required).
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration	1.0 mg/ml
General Readings	Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res. 1992 Mar;53(3):304-14. PubMed PMID: 1375818. Vos JH, van den Ingh TS, de Neijs M, van Mil FN, Ivanyi D, Ramaekers FC. Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord. Zentralbl Veterinarmed A. 1992 Dec;39(10):721-40. PubMed PMID: 1283472. Vos JH, van den Ingh TS, Ramaekers FC, Molenbeek RF, de Neijs M, van Mil FN, et al. The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region. Vet Pathol. 1993 Jul;30(4):352-61. PubMed PMID: 8212457. Bonfrer JM, Groeneveld EM, Korse CM, van Dalen A, Oomen LC, Ivanyi D. Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18. Tumour Biol. 1994;15(4):210-22. PubMed PMID: 7524130.
Storage	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
Format	Buffer System: PBS with 0.09% sodium azide as preservative. State: Liquid purified IgG fraction. Purified
Specificity	Specificity: This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. Species: Human, Canine, Feline, Zebrafish.
Gene ID	3875

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 18 (15 products)

Catalog No.	Species	Pres.	Purity	Source
TP760201	Cytokeratin 18 (full length, N-term HIS tag)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
					OriGene Technologies, Inc.
TP760377	Cytokeratin 18 (full length, N-term HIS tag, transcript variant 1)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
					OriGene Technologies, Inc.
AR09362PU-N	Cytokeratin 18
	Human	Purified	> 95 % pure (determined by SDS gelelectrophoresis)		0.25 mg / €520.00
					OriGene Technologies GmbH
AR09362PU-S	Cytokeratin 18
	Human	Purified	> 95 % pure (determined by SDS gelelectrophoresis)		0.1 mg / €360.00
					OriGene Technologies GmbH
AR10314PU-L	Cytokeratin 18
	Human	Purified	Greater than 95.0% as determined by SDS-PAGE	E. coli
					OriGene Technologies GmbH
AR10314PU-N	Cytokeratin 18
	Human	Purified	Greater than 95.0% as determined by SDS-PAGE	E. coli
					OriGene Technologies GmbH
AR10314PU-S	Cytokeratin 18
	Human	Purified	Greater than 95.0% as determined by SDS-PAGE	E. coli
					OriGene Technologies GmbH
PA1285	Cytokeratin 18
	Human	Purified	> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE	E. coli
					OriGene Technologies GmbH
PA1285X	Cytokeratin 18
	Human	Purified	> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE	E. coli
					OriGene Technologies GmbH
PA1285XC	Cytokeratin 18
	Human	Purified	> 95 % Purified by proprietary chromatographic techniques, purity is greater than 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE	E. coli
					OriGene Technologies GmbH
H00003875-P01-10	Cytokeratin 18
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00003875-P01-25	Cytokeratin 18
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00003875-P02	Cytokeratin 18
	Human	Purified
					Abnova Taiwan Corp.
H00003875-P02-10	Cytokeratin 18
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00003875-P02-25	Cytokeratin 18
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.

Positive controls for Cytokeratin 18 (3 products)

Catalog No.	Species
NBL1-12385	Cytokeratin 18 Lysate

		Novus Biologicals Inc.
NBL1-12386	Cytokeratin 18 Lysate

		Novus Biologicals Inc.
H00003875-T01	KRT18 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Cytokeratin 18 (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

Click here to see all secondary antibodies for Cytokeratin 18

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products

BM6007P Cytokeratin 18 antibody

Quick Overview