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BM6040P Reticulon 1 / RTN1 (Isoform RTN1-A/B) antibody

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0.1 mg / €370.00
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Mouse anti Human, Monkey, Rabbit Reticulon 1 / RTN1 RNL-3

BM6040P

Product Description for Reticulon 1 / RTN1

Mouse anti Human, Monkey, Rabbit Reticulon 1 / RTN1 RNL-3.
Properties: (Isoform RTN1-A/B)
Presentation: Purified
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Frozen Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for Reticulon 1 / RTN1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
Presentation Purified
Reactivity Hu, Mky, Rb
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone RNL-3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16799
Immunogen:
Small cell lung cancer cell line NCI-H82.
GeneID:
6252
Property Isoform RTN1-A/B
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoblotting: 1/100-1/500.
Immunocytochemistry.
Flow Cytometry: 1/100-1/200.
Immunohistochemistry on Frozen Sections: 1/100-1/200 with ABC as detection reagent.
Immunohistochemistry on Paraffin Sections: 1/100-1/200 with ABC as detection reagent.
Background Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon- 1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
Concentration 1.0 mg/ml
General Readings
  1. Broers JL, Mijnheere EP, Rot MK, Schaart G, Sijlmans A, Boerman OC, et al. Novel antigens characteristic of neuroendocrine malignancies. Cancer. 1991 Feb 1;67(3):619-33. PubMed PMID: 1845952.
  2. Roebroek AJ, van de Velde HJ, Van Bokhoven A, Broers JL, Ramaekers FC, Van de Ven WJ. Cloning and expression of alternative transcripts of a novel neuroendocrine-specific gene and identification of its 135-kDa translational product. J Biol Chem. 1993 Jun 25;268(18):13439-47. PubMed PMID: 7685762.
  3. Senden NH, van de Velde HJ, Broers JL, Timmer ED, Roebroek AJ, van de Ven WJ, et al. Cluster-10 lung-cancer antibodies recognize NSPs, novel neuro-endocrine proteins associated with membranes of the endoplasmic reticulum. Int J Cancer Suppl. 1994;8:84-8. PubMed PMID: 7515034.
  4. Senden NH, Timmer ED, Boers JE, van de Velde HJ, Roebroek AJ, Van de Ven WJ, et al. Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A. Eur J Cell Biol. 1996 Mar;69(3):197-213. PubMed PMID: 8900485.
  5. Senden NH, Timmer ED, de Bruïne A, Wagenaar SS, Van de Velde HJ, Roebroek AJ, et al. A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers. J Pathol. 1997 May;182(1):13-21. PubMed PMID: 9227337.
Storage Store the antibody undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
Phosphate buffered saline (PBS)
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Rabbit and Rhesus Monkey.
Specificity
Specificity:
RNL-3 recognizes an epitope located within the region of amino acids 421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B).
In normal tissues, RNL-3 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
Gene ID 6252

Accessory Products

Proteins and/or Positive Controls

Proteins for Reticulon 1 / RTN1 (4 products)

Catalog No. Species Pres. Purity   Source  

Reticulon 1 / RTN1 (transcript variant 1)
Reticulon 1 / RTN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Reticulon 1 / RTN1 (transcript variant 3)
Reticulon 1 / RTN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Reticulon 1 / RTN1
Reticulon 1 / RTN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Reticulon 1 / RTN1
Reticulon 1 / RTN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Reticulon 1 / RTN1 (1 products)

Catalog No. Species Pres. Purity   Source  

RTN1 overexpression lysate
RTN1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Recommended Secondary Antibodies for Reticulon 1 / RTN1 (11 products)

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Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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