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BM6049P M13 Bacteriophage G8P Capsid antibody

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0.1 mg / €370.00
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Mouse anti M13 Bacteriophage G8P Capsid RL-ph1

BM6049P

Product Description for M13 Bacteriophage G8P Capsid

Mouse anti M13 Bacteriophage G8P Capsid RL-ph1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for M13 Bacteriophage G8P Capsid

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Coat protein B, Gene 8 protein, Major coat protein
Presentation Purified
Applications C, E, F, ICC/IF, WB
Clonality Monoclonal
Clone RL-ph1
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P69541
Immunogen:
Isolated M13 phage coat proteins.
GeneID:
927333
Remarks:
Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse.
Isotype control AM03110PU-N, SM12P
Application Western Blot: 1/100-1/1000. 
Immunocytochemistry.
Flow Cytometry: 1/25-1/200.
Immunohistochemistry: 1/25-1/200 with ABC as detection system.
ELISA.
Affinity Chromatography.
Background The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities.
Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.
Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
Concentration 1.0 mg/ml
General Readings
  1. McCafferty J, Griffiths AD, Winter G, Chiswell DJ. Phage antibodies: filamentous phage displaying antibody variable domains. Nature. 1990 Dec 6;348(6301):552-4. PubMed PMID: 2247164.
  2. Clackson T, Hoogenboom HR, Griffiths AD, Winter G. Making antibody fragments using phage display libraries. Nature. 1991 Aug 15;352(6336):624-8. PubMed PMID: 1907718.
  3. Meulemans EV, Slobbe R, Wasterval P, Ramaekers FC, van Eys GJ. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol. 1994 Dec 9;244(4):353-60. PubMed PMID: 7527464.
  4. Meulemans EV, Nieland LJ, Debie WH, Ramaekers FC, van Eys GJ. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas. 1995;6(3):113-8. PubMed PMID: 8597626.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
RL-ph1 reacts with the M13 filamentous phage coat protein g8p with a Molecular Weight of approx. 5 kDa.
Gene ID 927333

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