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DM080P Neurofilament L (68kDa) (+ Neurofilament H) antibody

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0.1 mg / €370.00
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Quick Overview

Mouse anti Feline, Human, Mouse, Opossum, Rabbit, Rat Neurofilament L (68kDa) 2F11

DM080P

Product Description for Neurofilament L (68kDa)

Mouse anti Feline, Human, Mouse, Opossum, Rabbit, Rat Neurofilament L (68kDa) 2F11.
Properties: (+ Neurofilament H)
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Neurofilament L (68kDa)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L
Presentation Purified
Reactivity Fe, Hu, Ms, Op, Rb, Rt
Applications C, P, WB
Clonality Monoclonal
Clone 2F11
Host Mouse
Isotype IgG1
Molecular weight 68kDa and 200kDa (phosphorylated forms of these subunits).
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07196
Immunogen:
Human neurofilament preparation
GeneID:
4747
Property + Neurofilament H
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunoblotting. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Embedded Sections. 
Recommended Dilutions: 1/25–1/200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting.
Background Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation
Concentration 1 mg/ml
General Readings
  1. Klück P, van Muijen GN, van der Kamp AW, Tibboel D, van Hoorn WA, Warnaar SO, et al. Hirschsprung's disease studied with monoclonal antineurofilament antibodies on tissue sections. Lancet. 1984 Mar 24;1(8378):652-4. PubMed PMID: 6199635.
  2. van Muijen GN, Ruiter DJ, van Leeuwen C, Prins FA, Rietsema K, Warnaar SO. Cytokeratin and neurofilament in lung carcinomas. Am J Pathol. 1984 Sep;116(3):363-9. PubMed PMID: 6206727. (Free PMC Article available)
  3. Breckenridge LJ, Sommer IU, Blackshaw SE. Developmentally regulated markers in the postnatal cervical spinal cord of the opossum Monodelphis domestica. Brain Res Dev Brain Res. 1997 Oct 20;103(1):47-57. PubMed PMID: 9370059.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified antibody
Purified
Specificity
Specificity:
2F11 reacts exclusively with the phosphorylated isoform of the 70 kD neurofilament protein.
This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.
Species:
Human, Rabbit, Cat, Mouse, Rat and Opossum. Does not react with Dog.
Gene ID 4747

Accessory Products

Proteins and/or Positive Controls

Proteins for Neurofilament L (68kDa) (6 products)

Catalog No. Species Pres. Purity   Source  

Neurofilament L (68kDa)
Neurofilament L (68kDa) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa)
Lane 1: myosin (a), ß-galactosidase (b), phosphorylase B (c), BSA (d), ovalbumin (e). Lane 2: Mr 200 kD Neurofilament. Lane 3: Mr 160 kD Neurofilament. Bovine Purified > 98 % (determined by SDS gelelectrophoresis). Spinal Cord
0.25 mg / €670.00
  OriGene Technologies GmbH

Neurofilament L (68kDa)
Lane 1: myosin (a), ß-galactosidase (b), phosphorylase B (c), BSA (d), ovalbumin (e). Lane 2: Mr 200 kD Neurofilament. Lane 3: Mr 160 kD Neurofilament. Bovine Purified > 98 % (determined by SDS gelelectrophoresis). Spinal Cord
0.1 mg / €390.00
  OriGene Technologies GmbH

Neurofilament L (68kDa)
Neurofilament L (68kDa) Human
  Abnova Taiwan Corp.

Neurofilament L (68kDa)
Neurofilament L (68kDa) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Neurofilament L (68kDa)
Neurofilament L (68kDa) Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for Neurofilament L (68kDa) (2 products)

Catalog No. Species Pres. Purity   Source  

68kDa Neurofilament Light Lysate
Western Blot: 68kDa Neurofilament Light Lysate [NBL1-13577] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for NEFL
  Novus Biologicals Inc.

NEFL 293T Cell Transient Overexpression Lysate(Denatured)
NEFL 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Recommended Secondary Antibodies for Neurofilament L (68kDa) (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
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Mouse IgG (H+L chain) antibody
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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Click here to see all secondary antibodies for Neurofilament L (68kDa)
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