Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Cytokeratin 14

DM3066P Cytokeratin 14 antibody

See related secondary antibodies

Search for all "Cytokeratin 14"

1 ml / €260.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

View Datasheet

Quick Overview

Mouse anti Canine, Feline, Human, Porcine Cytokeratin 14 SPK-14

DM3066P

Product Description for Cytokeratin 14

Mouse anti Canine, Feline, Human, Porcine Cytokeratin 14 SPK-14.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for Cytokeratin 14

Product Category Primary Antibodies
Quantity 1 ml
Synonyms CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14
Presentation Supernatant
Reactivity Can, Fe, Hu, Por
Applications C, F, ICC/IF, WB
Clonality Monoclonal
Clone SPK-14
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02533
Immunogen:
Synthetic peptide corresponding to the carboxy-terminal sequence of Human Cytokeratin 14 coupled to keyhole limpet hemocyanin.
AA Sequence:
KVVSTHEQVLRTKN
GeneID:
3861
Application Immunocytochemistry.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Immunoblotting: 1/100-1/1000.
Flow Cytometry: 1/100-1/200.
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
General Readings
  1. Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res. 1992 Mar;53(3):304-14. PubMed PMID: 1375818.
  2. Bonfrer JM, Groeneveld EM, Korse CM, van Dalen A, Oomen LC, Ivanyi D. Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18. Tumour Biol. 1994;15(4):210-22. PubMed PMID: 7524130.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Feline, Human, Porcine, Canine.
Specificity
Specificity:
SPK-14 reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.
Gene ID 3861

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 14 (10 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 14 (full length, N-term HIS tag)
Cytokeratin 14 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Cytokeratin 14
Cytokeratin 14 Human Purified > 95 % determined by SDS gelelectrophoresis E. coli
0.25 mg / €580.00
  OriGene Technologies GmbH

Cytokeratin 14
Cytokeratin 14 Human Purified > 95 % determined by SDS gelelectrophoresis E. coli
0.1 mg / €390.00
  OriGene Technologies GmbH

Cytokeratin 14 (1-472, His-tag)
Cytokeratin 14 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,070.00
  OriGene Technologies GmbH

Cytokeratin 14 (1-472, His-tag)
Cytokeratin 14 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €400.00
  OriGene Technologies GmbH

Cytokeratin 14
Cytokeratin 14 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. 		E. coli
  OriGene Technologies GmbH

Cytokeratin 14
Cytokeratin 14 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. 		E. coli
  OriGene Technologies GmbH

Cytokeratin 14
Cytokeratin 14 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. 		E. coli
  OriGene Technologies GmbH

Cytokeratin 14
Cytokeratin 14 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Cytokeratin 14
Cytokeratin 14 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 14 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 14 Lysate
Western Blot: Cytokeratin 14 Lysate [NBL1-12381] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT14
  Novus Biologicals Inc.

KRT14 overexpression lysate
KRT14 overexpression lysate
  OriGene Technologies, Inc.

Recommended Secondary Antibodies for Cytokeratin 14 (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for Cytokeratin 14
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn