Product Description for Alpha-fetoprotein / AFP
Mouse anti Human Alpha-fetoprotein / AFP 057-11301.
Presentation: Purified
Product is tested for ELISA capture and detection, Enzyme Immunoassay.
Presentation: Purified
Product is tested for ELISA capture and detection, Enzyme Immunoassay.
Properties for Alpha-fetoprotein / AFP
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 1 mg |
| Synonyms | Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | E, E(caption)/E(detection) |
| Clonality | Monoclonal |
| Clone | 057-11301 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Add. information | Centrifuge before opening to ensure complete recovery of vial contents. |
| Application | ELISA. Recommended pair for sandwich immunoassay: Capture: AM00787PU-N Detection: AM00786PU-N |
| Background | Alpha fetoprotein is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults. Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down’s syndrome. |
| Concentration | 4.71 mg/ml (OD280nm, E0.1% = 1.4) |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 174 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Alpha-fetoprotein / AFP (14 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP306622 |
Alpha-fetoprotein / AFP |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| BA1024 |
Alpha-fetoprotein / AFP |
||||||
|
Human | Aff - Purified | > 95 % ≥95% pure by PAGE Electrophoresis and Western blot. | Cell culture |
1 mg /
€810.00
|
||
| OriGene Technologies GmbH | |||||||
| H00000174-P01-10 |
Alpha-fetoprotein / AFP |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00000174-P01-25 |
Alpha-fetoprotein / AFP |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00000174-Q01-10 |
Alpha-fetoprotein / AFP |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00000174-Q01-25 |
Alpha-fetoprotein / AFP |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P0398 |
Alpha-fetoprotein / AFP |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P0399 |
Alpha-fetoprotein / AFP |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5345 |
Alpha-fetoprotein / AFP |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P107-1 |
Alpha-fetoprotein / AFP |
||||||
|
Human | > 96 % | Amniotic Fluid | ||||
| BBI Solutions | |||||||
| P107-2 |
Alpha-fetoprotein / AFP |
||||||
|
Human | Extract | > 3 % | Amniotic Fluid | |||
| BBI Solutions | |||||||
| P109-3 |
Alpha-fetoprotein / AFP |
||||||
|
Human | Purified | > 95 % | Cord serum | |||
| BBI Solutions | |||||||
| 8F8 |
Alpha-fetoprotein / AFP |
||||||
|
Human | > 98 % | Cord serum |
1 mg /
€810.00
|
|||
| HyTest Ltd. | |||||||
| NBP1-74306 |
Alpha-fetoprotein / AFP |
||||||
|
Human | Purified | |||||
| Novus Biologicals Inc. | |||||||
Positive controls for Alpha-fetoprotein / AFP (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00000174-T01 |
AFP 293T Cell Transient Overexpression Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
| L006T6 |
AFP HEK293 Cell Transient Overexpression Lysate (Non-Denatured) |
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![]() |
Transient overexpression cell lysate was tested with Anti-AFP antibody (H00000174-M01) by Western Blots. | ||||||
| Abnova Taiwan Corp. | |||||||
| LY400455 |
AFP overexpression lysate |
||||||
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0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| NBL1-07375 |
Alpha 1 Fetoprotein Lysate |
||||||
![]() |
Protein | ||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Alpha-fetoprotein / AFP (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: Alpha 1 Fetoprotein Lysate [NBL1-07375] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for AFP.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Alpha%201%20Fetoprotein%20Lysate-Western%20Blot-NBL1-07375-img0001.jpg)
