Product Description for Apolipoprotein A I / APO AI
Mouse anti Human Apolipoprotein A I / APO AI 057-10029.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Radioimmunoassay.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Radioimmunoassay.
Properties for Apolipoprotein A I / APO AI
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 1 mg |
| Synonyms | APOA1, Apo-AI, ApoA-I, ApoAI |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | E, R |
| Clonality | Monoclonal |
| Clone | 057-10029 |
| Host | Mouse |
| Isotype | IgG2a |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Isotype control | AM03096PU-N |
| Add. information | Centrifuge before opening to ensure complete recovery of vial contents. |
| Application | This clone has been reported to be suitable for Immunohistochemistry on Paraffin Sections, Radioimmunoassay (RIA) and ELISA. |
| Background | Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases. |
| Concentration | 5.3 mg/ml (OD280nm, E0.1% = 1.4) |
| Storage | Store the antibody undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 335 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Apolipoprotein A I / APO AI (17 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP310762 |
Apolipoprotein A I / APO AI |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| TP720424 |
Apolipoprotein A I / APO AI |
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|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
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| OriGene Technologies, Inc. | |||||||
| TP723014 |
Apolipoprotein A I / APO AI |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
20 µg /
€135.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR00018PU-N |
Apolipoprotein A I / APO AI |
||||||
|
Hamster | Purified | Syrian hamster plasma, high density lipoproteins | Plasma |
0.1 mg /
€1,930.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09244PU-L |
Apolipoprotein A I / APO AI (25-267, His-tag) |
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|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.5 mg /
€820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09244PU-N |
Apolipoprotein A I / APO AI (25-267, His-tag) |
||||||
|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.1 mg /
€320.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09503PU-N |
Apolipoprotein A I / APO AI |
||||||
|
Human | > 97 % pure by SDS-PAGE gel and HPLC analyses. | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| AR09503PU-S |
Apolipoprotein A I / APO AI |
||||||
|
Human | > 97 % pure by SDS-PAGE gel and HPLC analyses. | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| AR31065PU-N |
Apolipoprotein A I / APO AI |
||||||
|
Human | Purified | > 90 % | Plasma |
0.5 mg /
€340.00
|
||
| OriGene Technologies GmbH | |||||||
| BA051 |
Apolipoprotein A I / APO AI |
||||||
|
Human | Purified |
> 99 % pure by SDS-PAGE. Purification: The protein was isolated by ultracentrifugal flotation between densities 1.063-1.21 g/ml, lyophilized then subjected to repeated Ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6 M Guanidine Hydrochloride -25 mM DTT. The product was then subjected to Sephacryl S200 filtration and eluted. |
Plasma |
1 mg /
€390.00
|
||
| OriGene Technologies GmbH | |||||||
| BA681 |
Apolipoprotein A I / APO AI |
||||||
|
Human | Purified | > 95 % pure by SDS-PAGE (Column Chromatography). | Plasma |
0.5 mg /
€260.00
|
||
| OriGene Technologies GmbH | |||||||
| BA1021 |
Apolipoprotein A I / APO AI |
||||||
|
Mouse | Purified | Purified from Swiss-Webster Mouse high density lipoprotein plasma fraction by gel-sieving- and Ion Exchange Column Chromatography | Plasma | |||
| OriGene Technologies GmbH | |||||||
| H00000335-P01-10 |
Apolipoprotein A I / APO AI |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00000335-P01-25 |
Apolipoprotein A I / APO AI |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P5326 |
Apolipoprotein A I / APO AI |
||||||
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5942 |
Apolipoprotein A I / APO AI |
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|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| NBP1-30197 |
Apolipoprotein A I / APO AI |
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|
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| Novus Biologicals Inc. | |||||||
Positive controls for Apolipoprotein A I / APO AI (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00000335-T01 |
Apolipoprotein A I (APO AI) Lysate(Denatured) |
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| Abnova Taiwan Corp. | |||||||
| NBL1-07609 |
Apolipoprotein A I Lysate |
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Protein | ||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for Apolipoprotein A I / APO AI (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
![Western Blot: Apolipoprotein A I Lysate [NBL1-07609] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for APOA1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Apolipoprotein%20A%20I%20Lysate-Western%20Blot-NBL1-07609-img0001.jpg)
