AM00800PU-N Apolipoprotein A I / APO AI antibody

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1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Apolipoprotein A I / APO AI 057-10029

AM00800PU-N

Product Description for Apolipoprotein A I / APO AI

Mouse anti Human Apolipoprotein A I / APO AI 057-10029.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Radioimmunoassay.

Properties for Apolipoprotein A I / APO AI

Product Category Primary Antibodies
Quantity 1 mg
Synonyms APOA1, Apo-AI, ApoA-I, ApoAI
Presentation Purified
Reactivity Hu
Applications E, R
Clonality Monoclonal
Clone 057-10029
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02647
Immunogen:
Human high density lipoprotein
GeneID:
335
Isotype control AM03096PU-N
Add. information Centrifuge before opening to ensure complete recovery of vial contents.
Application This clone has been reported to be suitable for Immunohistochemistry on Paraffin Sections, Radioimmunoassay (RIA) and ELISA.
Background Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease.
The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
Concentration 5.3 mg/ml (OD280nm, E0.1% = 1.4)
Storage Store the antibody undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
>90% pure (SDS-PAGE). Protein A chromatography
Buffer System:
10mM Phosphate, pH 7.4 containing 150mM Sodium chloride and 0,09% sodium azide
State:
Liquid purified IgG (0.2µm filtered)
Purified
Specificity
Specificity:
Recognizes Apolipoprotein A I.
This antibody does not cross react with Apo AII or Apo B in competitive RIA.
Aff. Const.:
5 x 10e6 L/m (determined by RIA followed by Scatchard plot analysis)
Species:
Human.
Gene ID 335

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein A I / APO AI (17 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €135.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Hamster Purified Syrian hamster plasma, high density lipoproteins Plasma
0.1 mg / €1,930.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI (25-267, His-tag)

Apolipoprotein A I / APO AI Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI (25-267, His-tag)

Apolipoprotein A I / APO AI Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 97 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 97 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 90 % Plasma
0.5 mg / €340.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 99 % pure by SDS-PAGE. 
Purification: The protein was isolated by ultracentrifugal flotation between densities 1.063-1.21 g/ml, lyophilized then subjected to repeated Ethanol extraction.
The precipitated protein was dried under nitrogen then dissolved in 6 M Guanidine Hydrochloride -25 mM DTT. The product was then subjected to Sephacryl S200 filtration and eluted.
Plasma
1 mg / €390.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 95 % pure by SDS-PAGE (Column Chromatography). Plasma
0.5 mg / €260.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Mouse Purified Purified from Swiss-Webster Mouse high density lipoprotein plasma fraction by gel-sieving- and Ion Exchange Column Chromatography Plasma
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

SDS-Page: Apolipoprotein A I Protein [NBP1-30197] - Apolipoprotein A-1, 30.3 kDa (264aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
  Novus Biologicals Inc.

Positive controls for Apolipoprotein A I / APO AI (2 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein A I (APO AI) Lysate(Denatured)

Apolipoprotein A I (APO AI) Lysate(Denatured)
  Abnova Taiwan Corp.

Apolipoprotein A I Lysate

Western Blot: Apolipoprotein A I Lysate [NBL1-07609] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for APOA1 Protein
  Novus Biologicals Inc.
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