HCV Envelope glycoprotein E2 (384-665) - AP00757PU-N

Product Description for HCV Envelope glycoprotein E2

Goat anti HCV Envelope glycoprotein E2.
Properties: (384-665)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for HCV Envelope glycoprotein E2

Product Category	Primary Antibodies
Quantity	1 ml
Synonyms	Hepatitis C Virus NS1, gp68, gp70
Presentation	Purified
Applications	E, ICC/IF, WB
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: Recombinant E2 (genotype 1a)
Property	384-665
Application	ELISA. Western Blot. Immunofluorescence.
Background	Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
Concentration	4-5 mg/ml (OD280nm, E0.1% = 1.4)
Product Citations	Purchased from Acris: Moon JS, Lee SH, Kim EJ, Cho H, Lee W, Kim GW, et al. Inhibition of Hepatitis C Virus in Mice by a Small Interfering RNA Targeting a Highly Conserved Sequence in Viral IRES Pseudoknot. PLoS One. 2016 Jan 11;11(1):e0146710. doi: 10.1371/journal.pone.0146710. eCollection 2016. PubMed PMID: 26751678. (Free PMC Article available, 6 images available)
General Readings	Lázaro CA, Chang M, Tang W, Campbell J, Sullivan DG, Gretch DR, et al. Hepatitis C virus replication in transfected and serum-infected cultured human fetal hepatocytes. Am J Pathol. 2007 Feb;170(2):478-89. PubMed PMID: 17255316. (Free PMC Article available, 11 images available)
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Sodium Sulfate Precipitation and Ion-Exchange Chromatography Buffer System: 0.01M PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and no stabilizing proteins State: Liquid purified Ig fraction (>95% pure) Purified
Specificity	Specificity: This antibody reacts with Hepatitis C Virus (HCV), E2 envelope protein.

Accessory Products

Recommended Secondary Antibodies for HCV Envelope glycoprotein E2 (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
				OriGene Technologies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
				OriGene Technologies GmbH
R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
				OriGene Technologies GmbH
R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
				OriGene Technologies GmbH