GAPDH - AM00847PU-N | acris-antibodies.com

Product Description for GAPDH

Mouse anti Bovine, Goat, Human, Porcine, Rabbit, Rat GAPDH B903M.
Presentation: Purified
Product is tested for ELISA (detection), Immunocytochemistry/Immunofluorescence.

Properties for GAPDH

Product Category	Primary Antibodies
Quantity	1 mg
Synonyms	CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
Presentation	Purified
Reactivity	Bov, Gt, Hu, Por, Rb, Rt
Applications	E(detection), ICC/IF
Clonality	Monoclonal
Clone	B903M
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P04406 Immunogen: Human GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice. GeneID: 2597
Isotype control	AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application	ELISA. Immunocytochemistry. Recommended pairs for Sandwich Immunoassay: Capture: AM00849PU-N Detection: AM00847PU-N
Background	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
Concentration	5.1 mg/ml (OD280nm, E0.1% = 1.4)
Storage	Store undiluted at 2-8°C. Shelf life: One year from despatch.
Format	Purification: Protein A Chromatography Buffer System: PBS, pH 7.4 Preservatives: 0.09% Sodium Azide State: Liquid purified IgG fraction (> 90% pure by SDS-PAGE) Purified
Specificity	Specificity: This antibody reacts with GAPDH. Negative Species: Dog, Cat, Mouse and Fish. Species: Human, Bovine, Porcine, Goat, Rabbit and Rat.
Gene ID	2597

Accessory Products

Proteins and/or Positive Controls

Proteins for GAPDH (17 products)

Catalog No.	Species	Pres.	Purity	Source
TP302309	GAPDH
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
AR09029PU-L	GAPDH (1-335)
	Human	Purified	> 95 % pure by SDS-PAGE	E. coli	0.5 mg / €820.00
					OriGene Technologies GmbH
AR09029PU-N	GAPDH (1-335)
	Human	Purified	> 95 % pure by SDS-PAGE	E. coli	0.1 mg / €320.00
					OriGene Technologies GmbH
H00002597-P01-10	GAPDH
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00002597-P01-25	GAPDH
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00002597-P02	GAPDH
	Human	Purified
					Abnova Taiwan Corp.
H00002597-P02-10	GAPDH
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00002597-P02-25	GAPDH
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.
					Abnova Taiwan Corp.
H00002597-Q01-10	GAPDH
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00002597-Q01-25	GAPDH
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
P4547	GAPDH
	Human
					Abnova Taiwan Corp.
8G4	GAPDH
	Human	Purified	> 98 %	Heart
					HyTest Ltd.
8G4r	GAPDH
	Rabbit	Purified	> 98 %	Heart
					HyTest Ltd.
NBC1-18528	GAPDH
			Protein
					Novus Biologicals Inc.
NBP1-74304	GAPDH
	Human	Purified
					Novus Biologicals Inc.
NBP1-74409	GAPDH
	Human	Purified
					Novus Biologicals Inc.
NBP1-74410	GAPDH
	Human	Purified
					Novus Biologicals Inc.

Positive controls for GAPDH (2 products)

Catalog No.	Species
H00002597-T01	GAPDH Lysate(Denatured)

		Abnova Taiwan Corp.
NBL1-10967	GAPDH Lysate

		Novus Biologicals Inc.

Recommended Secondary Antibodies for GAPDH (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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AM00847PU-N GAPDH antibody

Quick Overview

Product Description for GAPDH

Properties for GAPDH

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for GAPDH (17 products)

Positive controls for GAPDH (2 products)

Recommended Secondary Antibodies for GAPDH (11 products)

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