BM295 Clathrin heavy chain antibody

See related secondary antibodies

Search for all "Clathrin heavy chain"

0.1 mg / €290.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Amphibian, Bovine, Human, Porcine, Rat Clathrin heavy chain CHC5.9

BM295

Product Description for Clathrin heavy chain

Mouse anti Amphibian, Bovine, Human, Porcine, Rat Clathrin heavy chain CHC5.9.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Clathrin heavy chain

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker
Presentation Purified
Reactivity Amph, Bov, Hu, Por, Rt
Applications C, WB
Clonality Monoclonal
Clone CHC5.9
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P49951
Immunogen:
Coated vesicles (Clathrin) of Bovine brain.
GeneID:
281080
Isotype control SM13P
Application Immunohistochemistry on Frozen Tissue: 1/10.
(When reconstituted, dilute further with PBS, pH 7.4).
Incubation Time: 1 h at RT.
Immunoblotting (Western): 1/100-1/500 (ECL).
Successful use in Immunoprecipitation was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure.
Background Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.
General Readings
  1. Bruder G, Wiedenmann B. Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues. Exp Cell Res. 1986 Jun;164(2):449-62. PubMed PMID: 3709679.
  2. Hirst J and Robinson MS: Clathrin and adaptors. Biochim. Biophys. Acta 1404, 173-193 (1998).
  3. Qualmann B, Kessels MM, Kelly RB. Molecular links between endocytosis and the actin cytoskeleton. J Cell Biol. 2000 Sep 4;150(5):F111-6. PubMed PMID: 10974009. (Free PMC Article available, 1 images available)
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Gel filtration
State:
Lyophilized purified IgM fraction.
Purified
Reconstitution:
Restore with 1 ml distilled water.
Species Reactivity
Species reactivity (tested):
Human, Bovine, Pig, Rat and Amphibia.
Specificity
Specificity:
Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3).
Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues.
Structures and Tissues Specifically Detected:
Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries).
Reactivities on Cultured Cell Lines (tested so far):
HeLa, SV-40, RVF-SMC.
Gene ID 281080

Accessory Products

  • LinkedIn