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BM5043 Cytokeratin 6 antibody

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50 µg / €290.00
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Mouse anti Human, Mouse Cytokeratin 6 Ks6.KA12

BM5043

Product Description for Cytokeratin 6

Mouse anti Human, Mouse Cytokeratin 6 Ks6.KA12.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 6

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Cytokeratin 6, Cytokeratin6A, Cytokeratin6D, K6, KRT-6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E, KRTL1, Keratin type II cytoskeletal 6A
Presentation Purified
Reactivity Hu, Ms
Applications C, P, WB
Clonality Monoclonal
Clone Ks6.KA12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02538
Immunogen:
Keratin K6 of Human callus cytoskeletal preparation.
GeneID:
3853
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunoblotting (Western blot).
Immunohistochemistry on Frozen and Paraffin Embedded Sections after formalin fixation (microwave treatment improves performance). After reconstitution, dilute the antibody further 1/10 with PBS for Immunohistochemical applications.
Incubation Time: 1h at RT, extended with Paraffin.
Background Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation related keratins). Keratin 6 is found in hair follicles, neck squamous cell carcinomas, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
There are at least six isoforms of human type II keratin 6 (K6), K6A being the most abundant representing about 77% of all forms found in epithelia.
Product Citations
Purchased from Acris:
  1. Poschmann G, Sitek B, Sipos B, Ulrich A, Wiese S, Stephan C, et al. Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium. Mol Cell Proteomics. 2009 May;8(5):1105-16. doi: 10.1074/mcp.M800422-MCP200. Epub 2009 Jan 27. PubMed PMID: 19176476. (Free PMC Article available, 6 images available)
General Readings
  1. Wetzels RH, Kuijpers HJ, Lane EB, Leigh IM, Troyanovsky SM, Holland R, et al. Basal cell-specific and hyperproliferation-related keratins in human breast cancer. Am J Pathol. 1991 Mar;138(3):751-63. PubMed PMID: 1705754. (Free PMC Article available)
  2. Demirkesen C, Hoede N, Moll R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol. 1995 Dec;22(6):518-35. PubMed PMID: 8835171.
  3. Moll R. et al.: Differenzierungsmarker bei gynäkologischen Tumoren: Methodische und diagnostische Aspekte. In: Aktuelle Aspekte der Tumorimmunologie in der Gynäkologie. W. Zuckschwerdt Verlag (1995).
  4. Herrmann T, van der Hoeven F, Grone HJ, Stewart AF, Langbein L, Kaiser I, et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J Cell Biol. 2003 Jun 23;161(6):1105-15. PubMed PMID: 12821645. (Free PMC Article available, 6 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.5% BSA
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Restore in 1 ml distilled water.
Specificity
Specificity:
The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma.
Clone Ks6.KA12 represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions).
Reacts with a Mr 56000 polypeptide (keratin K6, formerly also designated cytokeratin 6) of squamous epithelia.
Tested Reactivities on Cultured Cell Lines: A-431, Detroit 562 and SCC-12.
Species:
Human and Mouse.
Gene ID 3853

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 6 (6 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 6 (full length, N-term HIS tag)
Cytokeratin 6 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Cytokeratin 6
Cytokeratin 6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Cytokeratin 6
Cytokeratin 6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Cytokeratin 6
Cytokeratin 6 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Cytokeratin 6
Cytokeratin 6 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Cytokeratin 6
Cytokeratin 6 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Positive controls for Cytokeratin 6 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 6a Lysate
Western Blot: Cytokeratin 6a Lysate [NBL1-12394] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for KRT6A
  Novus Biologicals Inc.

KRT6A overexpression lysate
KRT6A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Recommended Secondary Antibodies for Cytokeratin 6 (11 products)

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Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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Click here to see all secondary antibodies for Cytokeratin 6
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