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BM5152P basic Cytokeratin antibody

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50 µg / €310.00
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Quick Overview

Mouse anti Amphibian, Bovine, Human, Rat basic Cytokeratin Ks pan 1-8

BM5152P

Product Description for basic Cytokeratin

Mouse anti Amphibian, Bovine, Human, Rat basic Cytokeratin Ks pan 1-8.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for basic Cytokeratin

Product Category Primary Antibodies
Quantity 50 µg
Presentation Purified
Reactivity Amph, Bov, Hu, Rt
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone Ks pan 1-8
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Cytoskeletal proteins from cultured Human MCF-7 cells
Isotype control AM03096PU-N
Application Immunoblotting.
Immunofluorescence.
Immunohistochemistry on Frozen and cytological material.
Working Dilution: 1/10 with PBS, pH 7.4 for Immunohistochemistry.
Incubation Time: 1h at RT.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
General Readings
  1. Achtstätter T, Fouquet B, Rungger-Brändle E, Franke WW. Cytokeratin filaments and desmosomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species. Differentiation. 1989 May;40(2):129-49. PubMed PMID: 2474473.
  2. Gould VE, Koukoulis GK, Jansson DS, Nagle RB, Franke WW, Moll R. Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast. Am J Pathol. 1990 Nov;137(5):1143-55. PubMed PMID: 1700618. (Free PMC Article available)
  3. Heid HW, Moll I, Franke WW. Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation. 1988;37(2):137-57. PubMed PMID: 2456239.
  4. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379.
  5. Moll R, Schiller DL, Franke WW. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell Biol. 1990 Aug;111(2):567-80. PubMed PMID: 1696264. (Free PMC Article available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Final solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in 1 ml distilled water
Species Reactivity
Species reactivity (tested):
Human, Bovine, Rat, Amphibia.
Specificity
Specificity:
This antibody represents an excellent marker for distinguishing carcinomas from non-epithelial tumors.
Polypeptides Reacting: Mr 52 500-Mr 68 000 keratins (type II keratins K1-K8; formerly also designated Cytokeratins 1-8) of Human epithelial cells.
Tumors Specifically Detected: All epithelium-derived neoplasms.
Tested Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, Bovine BMGE+H, BMGE-H, MDBK.

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