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BM5505 Neurofilament M (160 kD) antibody

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1 ml / €360.00
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Mouse anti Bovine, Guinea Pig, Human, Rat Neurofilament M (160 kD) NF403

BM5505

Product Description for Neurofilament M (160 kD)

Mouse anti Bovine, Guinea Pig, Human, Rat Neurofilament M (160 kD) NF403.
Presentation: Supernatant
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Neurofilament M (160 kD)

Product Category Primary Antibodies
Quantity 1 ml
Synonyms (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide
Presentation Supernatant
Reactivity Bov, GP, Hu, Rt
Applications C, P, WB
Clonality Monoclonal
Clone NF403
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07197
Immunogen:
Neurofilament preparation isolated from calf brain tissue.
GeneID:
4741
Application Immunohistochemistry on Frozen and Paraffin Sections: 1/10 for 1 h at room temperature.
Recommended Positive Control: Human brain tissue.
Immunoblotting.
Cell preparations.
Background Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. This antibody was raised against the C-terminal region of NF-M, a region highly conserved between NF-M molecules in different species. Accordingly the antibody recognizes all mammalian and avian NF-M molecules. The name NF-M refers to the fact that this is the "middle" or "medium" protein of neurofilament triplet, the other two proteins being "low" or "light" (NF-L) and "high" or "heavy" (NF-H). NF-M is sometimes also referred to as NEF3 or Neurofilament
General Readings 1. Hacker GW et al: Antibodies to neurofilament protein and other brain proteins reveal the innervation of peripheral organs.
Immunochemistry 82, 581-593 (1985)
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
State:
Liquid Culture Supernatant containing 0.09% Sodium Azide as preservative.
Supernatant
Specificity
Specificity:
Marker for the differentiation of 160 kD neurofilament peptide from other intermediate filament proteins.
The antibody is useful for studying neurofilament expression and for tumor characterization (pheochromocytoma, paraganglioma, ganglioneuroblastoma, ganglioneuroma and other tumors of neuronal origin).
Species:
Human, Bovine, Rat and Guinea Pig.
Gene ID 4741

Accessory Products

Proteins and/or Positive Controls

Proteins for Neurofilament M (160 kD) (1 products)

Catalog No. Species Pres. Purity   Source  

Neurofilament M (160 kD) (transcript variant 1)
Neurofilament M (160 kD) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

Positive controls for Neurofilament M (160 kD) (1 products)

Catalog No. Species Pres. Purity   Source  

160kDa Neurofilament Medium Lysate
Western Blot: 160kDa Neurofilament Medium Lysate [NBL1-13578] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for NEFM
  Novus Biologicals Inc.

Recommended Secondary Antibodies for Neurofilament M (160 kD) (11 products)

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Mouse IgG (H+L chain) antibody
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
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Mouse IgG (H+L chain), adsorbed antibody
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