BM5542 SLCO1B1 / OATP2 antibody

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5 ml / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human SLCO1B1 / OATP2 mESL

BM5542

Product Description for SLCO1B1 / OATP2

Mouse anti Human SLCO1B1 / OATP2 mESL.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for SLCO1B1 / OATP2

Product Category Primary Antibodies
Quantity 5 ml
Synonyms LST-1, LST1, Liver-specific organic anion transporter 1, OATP-2, OATP-C, OATPC, SLC21A6, Sodium-independent organic anion-transporting polypeptide 2, Solute carrier family 21 member 6, Solute carrier organic anion transporter family member 1B1
Presentation Supernatant
Reactivity Hu
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone mESL
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y6L6
Immunogen:
Synthetic C-terminus (21 aa) of Human organic anion transporter SLC21A6 coupled to KLH
GeneID:
10599
Application Immunoprecipitation.
Immunofluorescence Microscopy (Ready-to-use).
Incubation Time: 1 h at RT).
Western blotting: for optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples should be kept at 37°C for 30 min (cf. Cui et al.).
Background The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATPC, and LST1.
General Readings
  1. Cui Y, König J, Nies AT, Pfannschmidt M, Hergt M, Franke WW, et al. Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma. Lab Invest. 2003 Apr;83(4):527-38. PubMed PMID: 12695556.
  2. Hagenbuch B, Meier PJ. The superfamily of organic anion transporting polypeptides. Biochim Biophys Acta. 2003 Jan 10;1609(1):1-18. PubMed PMID: 12507753.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
State:
Liquid Culture Supernatant containing 0.09% Sodium Azide
Supernatant
Specificity
Specificity:
The antibody reacts with OATP2/OATP1B1, Human organic anion transporter SLC21A6 (90 kD).
It recognizes SCL21A6 transfected HEK293 cells, negative with HepG2 and PLC (cultured cell lines tested so far).
Using Immunofluorescence Microscopy mESL stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma and the human cell line HEK293 transfected with SLC21A6.
In Western blot analyses the monoclonal antibody mESL reacts selectively with the 90 kD protein (after SDS-PAGE) present in the basolateral membrane fractions of human liver. According to a recent new nomenclature this hepatocytespecific protein is identical to OATP1B1 (see Ref. 2) also known as OATP-C.
Species:
Human.
Gene ID 10599

Accessory Products

Proteins and/or Positive Controls

Proteins for SLCO1B1 / OATP2 (2 products)

Catalog No. Species Pres. Purity   Source  

SLCO1B1 / OATP2

SLCO1B1 / OATP2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SLCO1B1 / OATP2

SLCO1B1 / OATP2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for SLCO1B1 / OATP2 (1 products)

Catalog No. Species Pres. Purity   Source  

OATP2 Lysate

Western Blot: OATP2 Lysate [NBL1-16203] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SLCO1B1
  Novus Biologicals Inc.
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