Product Description for Plectin
Guinea Pig anti Fish, Human, Xenopus Plectin.
Properties: (C-term)
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.
Properties: (C-term)
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.
Properties for Plectin
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | Hemidesmosomal protein 1, PCN, PLEC, PLEC1, PLTN, Plectin-1 |
| Presentation | Serum |
| Reactivity | Fi, Hu, Xen |
| Applications | C, ICC/IF, P, WB |
| Clonality | Polyclonal |
| Host | Guinea Pig |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Property | C-term |
| Application | Western blot (1/16,000 using ECL). Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/500). Incubation Time: 1 h at RT, extended with paraffin sections; Microwave treatment enhances staining on paraffin sections. |
| Background | Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking. Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes. Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions. |
| General Readings |
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| Storage | Store undiluted at 2-8°C. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 5339 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Plectin (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00005339-Q01-10 |
Plectin |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00005339-Q01-25 |
Plectin |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Plectin (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1322AP |
Guinea pig IgG (H+L chain) antibody |
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|
|
Rabbit | AP | |||||
| OriGene Technologies GmbH | |||||||
| R1322F |
Guinea pig IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| OriGene Technologies GmbH | |||||||
| R1322HRP |
Guinea pig IgG (H+L chain) antibody |
||||||
|
|
Rabbit | HRP | GP | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1322P |
Guinea pig IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Aff - Purified | |||||
| OriGene Technologies GmbH | |||||||
| R1322T |
Guinea pig IgG (H+L chain) antibody |
||||||
|
|
Rabbit | TRITC | |||||
| OriGene Technologies GmbH | |||||||
| R1436C2 |
Guinea pig IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1436C3 |
Guinea pig IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1436C5 |
Guinea pig IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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