BP5046 Gastrin antibody

Swiss Prot Num:

P01350

Immunogen:

Synthetic Human Gastrin I conjugated to BSA

GeneID:

2520

Purchased from Acris:

1. Fischer T, Doll C, Jacobs S, Kolodziej A, Stumm R, Schulz S. Reassessment of sst2 somatostatin receptor expression in human normal and neoplastic tissues using the novel rabbit monoclonal antibody UMB-1. J Clin Endocrinol Metab. 2008 Nov;93(11):4519-24. doi: 10.1210/jc.2008-1063. Epub 2008 Aug 12. PubMed PMID: 18697876.
2. Haid D, Widmayer P, Breer H. Nutrient sensing receptors in gastric endocrine cells. J Mol Histol. 2011 Aug;42(4):355-64. doi: 10.1007/s10735-011-9339-1. Epub 2011 Jul 13. PubMed PMID: 21750971.
3. Haid DC, Jordan-Biegger C, Widmayer P, Breer H. Receptors responsive to protein breakdown products in g-cells and d-cells of mouse, swine and human. Front Physiol. 2012 Apr 10;3:65. doi: 10.3389/fphys.2012.00065. eCollection 2012. PubMed PMID: 22514536.
4. Widmayer P, Goldschmid H, Henkel H, Küper M, Königsrainer A, Breer H. High fat feeding affects the number of GPR120 cells and enteroendocrine cells in the mouse stomach. Front Physiol. 2015 Feb 27;6:53. doi: 10.3389/fphys.2015.00053. eCollection 2015. PubMed PMID: 25774135. 
5. Lang, K;Breer, H;Frick, C. Mechanosensitive ion channel Piezo1 is expressed in antral G cells of murine stomach. Cell Tissue Res. 2017. PubMed PMID: 29264643.

1. Portela-Gomes GM, Stridsberg M, Johansson H, Grimelius L. Complex co-localization of chromogranins and neurohormones in the human gastrointestinal tract. J Histochem Cytochem. 1997 Jun;45(6):815-22. PubMed PMID: 9199667.
2. Mulder H, Lindh AC, Ekblad E, Westermark P, Sundler F. Islet amyloid polypeptide is expressed in endocrine cells of the gastric mucosa in the rat and mouse. Gastroenterology. 1994 Sep;107(3):712-9. PubMed PMID: 8076756.

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Preservatives:

0.09% Sodium Azide

State:

Lyophilized Serum
Serum

Reconstitution:

Dissolve the antiserum in 50-100 µl distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.09% Sodium Azide.

Species reactivity (tested):

Human and Rat.

Specificity:

Gastrin-secreting cells are numerous in the antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of Gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum.
Absorption with 10-100 µg Gastrin 1-34 and CCK 8 per ml antiserum abolishes the staining.