Product Description for Collagen type II alpha 1 chain
Rabbit anti Bovine, Human, Mouse, Rat, Sheep Collagen type II alpha 1 chain.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties for Collagen type II alpha 1 chain
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.5 mg |
| Synonyms | Alpha-1 type II collagen, COL2A1 |
| Presentation | Purified |
| Reactivity | Bov, Hu, Ms, Rt, Sh |
| Applications | C, E, IP, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Application | Anti-Collagen antibodies have been used for Indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and Western blotting for highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/400. QC: This product was assayed by Immunoblot and found to be reactive against Collagen II at a dilution of 1/5,000 to 1/10,000. This product was also assayed against 1.0 µg of Collagen II in a standard Sandwich ELISA using Peroxidase conjugated affinity purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/8,000 of the stock concentration showed best results. |
| Background | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. For immunization collagens from human and bovine placenta and cartilage extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Product Citations |
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| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. For extended storage, mix with an equal volume of glycerol. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 1280 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Collagen type II alpha 1 chain (6 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| RA003 |
Collagen type II alpha 1 chain (Adult Knee Cartilage) |
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|
Human | > 98 % Chromatographically and immunologically pure. | Cartilage |
0.1 mg /
€740.00
|
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| OriGene Technologies GmbH | |||||||
| H00001280-P01-10 |
Collagen type II alpha 1 chain |
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|
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001280-P01-25 |
Collagen type II alpha 1 chain |
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|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P0384 |
Collagen type II alpha 1 chain |
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Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4916 |
Collagen type II alpha 1 chain |
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Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4917 |
Collagen type II alpha 1 chain |
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Human | ||||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Collagen type II alpha 1 chain (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
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|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
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|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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